Description
Protein Chromatography (2nd Ed., 2nd ed. 2017)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1485
Coordinators: Walls Dermot, Loughran Sinéad T.
Language: EnglishSubject for Protein Chromatography:
Keywords
protein stability and storage; avoiding proteolysis during chromatography; protein quantitation; immuno-qPCR; purification of recombinant proteins; recombinant antibody production; high-throughput systems; physicochemical properties of the target protein; ion-exchange; immunoaffinity chromatography
Support: Print on demand
Support: Print on demand
Description
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This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.
1. A Synopsis of Protein and their Purification
Dermot Walls, Gary Cooney, and Sinéad T. Loughran
2. Gel-Filtration Chromatography
3. Immunoaffinity Chromatography: Concepts and Applications
Jenny Fitzgerald, Paul Leonard, Elaine Darcy, Shikha Sharma, and Richard O’Kennedy
4. Avoiding Proteolysis During Protein Purification
5. Scale-Up of Protein Purification: Downstream Processing Issues
6. Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources
7. Protein Stability: Enhancement and Measurement
8. Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
Part II Protocols Chapters
9. Storage and Lyophilisation of Pure Proteins
10. Differential Precipitation and Solubilization of Proteins
Barry J. Ryan and Gemma K. Kinsella
11. Ion-Exchange Chromatography: Basic Principles and Application
12. Protein Quantitation and Analysis of Purity
13. Purification of Proteins Fused to Maltose-Binding Protein
14. Purification of Poly-Histidine-Tagged Proteins
15. Purification of Antibodies Using Affinity Chromatography
Elaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, and Richard O’ Kennedy
16. Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens
17. Measuring Protein-Protein Interactions Using Biacore
18. Hydrophobic Interaction Chromatography
19. Fast Protein Liquid Chromatography
20. Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems
21. Strategies for the Purification of Membrane Proteins
22. Antimicrobial Peptide Production and Purification
23. Lectin Affiity Chromatography (LAC)
< ` Brendan F. O’Connor, Donal Monaghan, and Jonathan Cawley