Description
Protein Downstream Processing (2nd Ed., 2nd ed. 2021)
Design, Development, and Application of High and Low-Resolution Methods
Methods in Molecular Biology Series, Vol. 2178
Coordinator: Labrou Nikolaos E.
Language: EnglishSubjects for Protein Downstream Processing:
Publication date: 11-2021
511 p. · 17.8x25.4 cm · Paperback
Publication date: 11-2020
Support: Print on demand
Description
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Preface…
Table of Contents…
Contributing Authors…
Part I Screening and Design Purification Strategies
1. Protein Purification Technologies
Nikolaos E. Labrou
2. High Throughput Process Development: I. Process Chromatography
Anurag S. Rathore and R. Bhambure
3. High Throughput Process Development: II. Membrane Chromatography
Anurag S. Rathore and S. Muthukumar
4. Media Selection in Ion Exchange Chromatography in a Single Microplate
Charlotte Cabanne and Xavier Santarelli
5. High Throughput Screening of Dye-Ligands for Chromatography
Sunil Kumar and Narayan S. Punekar
6. Technical Optimizations for the High Throughput Purification of Antibodies on Automated Liquid Handlers
Peter M. Schmidt
Part II Low-Resolution Protein Purification Methods
7. Aqueous Two-Phase Systems for Clean-Up and Recovery of Enzymes from Plants and Plant-Derived Extracts
Oscar Aguilar, Erick Heredia-Olea, Esther Perez-Carrillo, and Marco Rito-Palomares
8. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins
Anurag S. Rathore and R. Bhambure
9. Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments
Ario de Marco
Part III High-Resolution Protein Purification Methods
10. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview
Ana Sofia Pina, Íris L. Batalha, Ana M. G. C. Dias, and Ana Cecília A. Roque
11. Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins
Sharon Williams, Phil Morton, and Dev Baines
12. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery
My Hedhammar, Johan Nilvebrant, and Sophia Hober
13. An Orthogonal Fusion Tag for Efficient Protein Purification
Johan Nilvebrant, Mikael Åstrand, and Sophia Hober
14. Biomimetic Affinity Ligands for Protein Purification
Isabel T. Sousa and M. Ângela Taipa
15. Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification
Evangelia G. Chronopoulou, Georgios Premetis, Christina Varotsou, Nikolaos Georgakis, Elisavet Ioannou and Nikolaos E. Labrou
16. Design of Affinity Chromatography Peptide Ligands through Combinatorial Peptide Libraries Screening
G. R. Barredo, S. L. Saavedra, M. C. Martínez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, O. Cascone, and S. A. Camperi
17. ZCa: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification
Julia Scheffel, Sara Kanje, and Sophia Hober
18. Macroporous Polymer Monoliths for Affinity Chromatography and Solid Phase Enzyme Processing
E.G. Korzhikova-Vlakha, G.A. Platonovaa, and T.B. Tennikova
19. Sample Displacement Batch Chromatography of Proteins
Laura Heikaus, Siti Hidaya, Manasia Gaikwad, Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlüter
20. Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3
Salvatore G. De-Simone and D. William Provance, Jr.
21. Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins
Sanhita Maity, Musaab Al-ameer, Ravi Kumar Gundampati, Shilpi Agrawal, and Thallapuranam Krishnaswamy Suresh Kumar
22. Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+
Jessica J. Gomez-Lugo, Bryan D. Santos, David A. Perez-Perez, Jorge M. Montfort-Gardeazabal, Megan M. McEvoy, and Xristo Zarate
23. Antibody Aggregate Removal using a Mixed-Mode Chromatography Resin
Tao Chen, Gaili Guo, Guoqing Tan, Ying Wang, and Yifeng Li
Part IV Assessing Protein Structural Integrity, Purity, and Stabilization
24. Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry
Cosima D. Calvano, Mariachiara Bianco, Ilario Losito, and Tommaso R.I. Cataldi
25. Protein Structure Analysis and Validation with X-Ray Crystallography
Anastassios C. Papageorgiou, Nirmal Poudel, and Jesse Mattsson
26. Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes
Annette C. Moser, Benjamin White, and Frank A. Kovacs
27. Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin
Pavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, and Eduarda Dráberová
Part V Applications/Case Studies
28. G Protein-Coupled Receptor Expression and Purification
Karolina Corin, Lotta T. Tegler, and Sotirios Koutsopoulos
29. (Hyper)thermophilic Enzymes: Production and Purification
Pierpaolo Falcicchio, Mark Levisson, Servé W.M. Kengen, Sotirios Koutsopoulos, and John van der Oost
30. Screening of Recombinant Lignocellulolytic Enzymes through Rapid Plate Assays
Anthi Karnaouri, Anastasia Zerva, Paul Christakopoulos, and Evangelos Topakas
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts