Protein Misfolding and Cellular Stress in Disease and Aging, 2010 Concepts and Protocols Methods in Molecular Biology Series, Vol. 648

How and why certain proteins misfold and how this misfolding is linked to many disease processes has become a well-documented topic of study. Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols moves beyond the basics to emphasize the molecular effects of protein misfolding at a cellular level, to delineate the impacts and cellular reactions that play a role in pathogenetic mechanisms, and to pinpoint possible manipulations and treatment strategies that can counteract, modify, or delay the consequences of misfolding. The volume begins with several concepts and approaches developed in the recent past including a connection to the research field of aging, where protein misfolding diseases have been equated to premature aging processes, and the book?s coverage continues with detailed descriptions of protocols for relevant experimental approaches. Written in the highly successful Methods in Molecular Biology? series format, protocols chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols aims to aid researchers in the field, as well as medical professionals and molecular biologists, in shaping and performing research related to this intriguing and vital subject.

Concepts.- Protein Misfolding and Cellular Stress: An Overview.- Protein Aggregation Diseases: Toxicity of Soluble Prefibrillar Aggregates and Their Clinical Significance.- Consequences of Stress in the Secretary Pathway: The ER Stress Response and Its Role in the Metabolic Syndrome.- What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases?.- Autophagy in Disease.- Mitophagy and Mitoptosis in Disease Processes.- Cellular Stress and Protein Misfolding During Aging.- Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques.- Protocols.- Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase System.- Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress.- Examining Ubiquitinated Protein Aggregates in Tissue Sections.- Determination of Proteasomal Activities.- Measurement of Autophagy in Cells and Tissues.- Transcription Factor Sequestration by Polyglutamine Proteins.- Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity.- Measurement of Mitochondrial ROS Production.- In Vivo Detection of Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria.- Measurement of Oxidized/Reduced Glutathione Ratio.- Determination of Altered Mitochondria Ultrastructure by Electron Microscopy.- Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of Mitochondrial Permeability Transition-Dependent Toxicity.- A Cellular Viability Assay to Monitor Drug Toxicity.- Rescue of Misfolded Proteins and Stabilization by Small Molecules.

Gives an overview of cellular stress defense systems Provides state of the art technologies in molecular cell biology Details methods to determine oxidative stress Includes supplementary material: sn.pub/extras