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Proteomics for Biomarker Discovery, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1959

Langue : Anglais
Couverture de l’ouvrage Proteomics for Biomarker Discovery
This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Proteomics for Biomarker Discovery: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Pre- and Post-Analytical Factors in Biomarker Discovery

            Frank Klont, Peter Horvatovich, Natalia Govorukhina, and Rainer Bischoff

 

2. Pre-Fractionation of Non-Circulating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers

            Annarita Farina

 

3. Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers

            Rosana Navajas, Fernando J. Corrales, and Alberto Paradela

 

4. Protein Biomarker Discovery Using Human Blood Plasma Microparticles

            Raghda Saad Zaghloul Taleb, Pacint Moez, Doreen Younan, Martin Eisenacher, Matthias Tenbusch, Barbara Sitek, and Thilo Bracht

 

5. A Standardized and Reproducible Proteomics Protocol for Bottom-Up Quantitative Analysis of Protein Samples Using SP3 and Mass Spectrometry

            Christopher S. Hughes, Poul H. Sorensen, and Gregg B. Morin

 

6. Analyzing Cerebrospinal Fluid Proteomes to Characterize Central Nervous System Disorders: A Highly Automated Mass Spectrometry-Based Pipeline for Biomarker Discovery

            Antonio Núñez Galindo, Charlotte Macron, Ornella Cominetti, and Loïc Dayon

 

7. Lys-C/Trypsin Tandem-Digestion Protocol for Gel-Free Proteomic Analysis of Colon Biopsies

            Armin Schniers, Yvonne Pasing, and Terkel Hansen

 

8. Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics

            Leslie Muller, Luc Fornecker, Sarah Cianferani, and Christine Carapito

 

9. Protein Biomarker Discovery in Non-Depleted Serum by Spectral Library-Based Data-Independent Acquisition Mass Spectrometry

            Alexandra Kraut, Mathilde Louwagie, Christophe Bruley, Christophe Masselon, Yohann Couté, Virginie Brun, and Anne-Marie Hesse

 

10. Discovering Protein Biomarkers from Clinical Peripheral Blood Mononuclear Cells Using Data-Independent Acquisition Mass Spectrometry

            Xin Ku and Wei Yan

 

11. Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid

            Jérôme Vialaret, Sylvain Lehmann, and Christophe Hirtz

 

12. Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions

            Didia Coelho Graça, Ralf Hartmer, Wolfgang Jabs, Alexander Scherl, Lorella Clerici, Kaveh Samii, Yury O. Tsybin, Denis Hochstrasser, and Pierre Lescuyer

 

13. Development of a Highly Multiplexed SRM Assay for Biomarker Discovery in Formalin-Fixed Paraffin-Embedded Tissues

            Carine Steiner, Pierre Lescuyer, Jean-Christophe Tille, Paul Cutler, and Axel Ducret

 

14. Development and Validation of Multiple Reaction Monitoring (MRM) Assays for Clinical Applications

            Georgia Kontostathi, Manousos Makridakis, Vasiliki Bitsika, Nikolaos Tsolakos, Antonia Vlahou, and Jerome Zoidakis

 

15. Protein-Level Statistical Analysis of Quantitative Label-Free Proteomics Data with ProStaR

            Samuel Wieczorek, Florence Combes, Hélène Borges, and Thomas Burger

 

16. Computation and Selection of Optimal Biomarker Combinations by Integrative ROC Analysis using CombiROC

            Mauro Bombaci and Riccardo L. Rossi

 

17. PanelomiX for the Combination of Biomarkers

            Xavier Robin

 

18. Designing an In Silico Strategy to Select Tissue-Leakage Biomarkers Using the Galaxy Framework

            Lien Nguyen, Virginie Brun, Florence Combes, Valentin Loux, and Yves Vandenbrouck

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains implementation advice from the experts

Date de parution :

Ouvrage de 292 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 189,89 €

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