Rapid Cycle Real-Time PCR — Methods and Applications, Softcover reprint of the original 1st ed. 2004
Quantification

Methods: Part 1: Onno Bakker, Academic Medical Centre Amsterdam, NL - Housekeeping Genes: A Gold Standard?- Part 2: Weisser/ Schnittger, Klinikum Grosshadern München, Germany - The choice of house keeping genes in MRD-quantification of t(8;21) positive AML.- Part 3: Ronald H. Lekanne-Deprez, Dep. of Anatomie & Embryologie, Amsterdam, The Netherlands - Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative for the Standard Curve Approach.- Part 4: Jochen Wilhelm, University Giessen - Estimation of Genome Sizes by Quantitative Real-Time PCR; Applications; Regulation and Development.- Part 5: N. Neubauer, University of Copenhagen, Biokemisk Afd., Copenhagen, Denmark - Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I.- Part 6: Jürgen Loeffler, Medizinische Klinik, Abt. II, Otfried-Müller-Str. 10, 72076 Tübingen, Germany - Quantification of T-Cell Receptor Excision Circle DANN Using Fluorescence Resonance Energy Transfer and the LightCycler System.- Part 7: Jim Whelan, Plant Molecular Biology Group, University of Western Australia, Crawley, Australia - Investigation of Mitochondrial Biogenesis in Plants using Quantitative Real-Time PCR.- Part 8: E. Veistinen, Turku University, Dept. Medical Microbiology, Kiinamyllynkatu 13, FIN 20520 Turku - Quantification of Ikaros Family Isoforms by Real-Time PCR.- Part 9: P. Stordeur, Dep. Immunologie-Hematologie-Transfusion, Hopital Erasme, Brussels, Belgium - Methods to quantify cytokine gene expression by Real-Time PCR; Oncology.- Part 10: Dr. Bernard, Idahotech, Salt Lake City, USA - Quantitative profiling for breast cancer using DNA and RNA markers.- Part 11: Melanie Königshoff, University Giessen - Quantification of HER-2/NEU Gene CopyNumber in Breast Cancer Tissue.- Part 12: Remedios Castelló Cros, Dpto. Bioquímica. Centro de Investigación, Hospital la Fe, Av. Campanar, 21, 46009 Valencia, Spain - Quantitative real-time reverse transcription-PCR assay for urokinase plasminogen activator, plasminogen activator inhibitor type 1, and tissue metalloproteinase inhibitor type 1 gene expressions in primary breast cancer.- Part 13: C. H. W. Klaasen, C. Wilhelmina Hospital, Dep. Of Med. Microbiology & Infectious Diseases Nijmegen, NL - Relative Quantification of Human DNA in Feaces (stool).- Part 14: Chung-Che (Jeff) Chang, Assistant Professor, Director, Hematopathology Fellowship and Molecular/Pharmacogenetics Lab., Dep. of Pathology, Medical College of Wisconsin, 9200 W. Wisconsin Ave., Milwaukee, WI 53226 - Real-time quatification of tumor load (t(14;18))in follicular lymphoma patients.- Part 15: P. Bolufer, Laboratorio de Biologia Molecular, Universitario La Fe, Valencia, Spain - Real time quantification of AML rearrangements (AML1/ETO and TEL/AML1 ) in the diagnosis and monitoring of acute leukemia; Genetics.- Part 16: Francisco Barros, INGO, Santiago de Compostela - Gene Dosage Determination by Real Time PCR.- Part 17: Elaine Lyon, ARUP Laboratories, Salt Lake City, USA - Deletions and duplications of the cytochrome p450 2D6 gene using a reference gene and competitor (Alison Millson).- Part 18: Karin Berg, Pathology, John Hopkins Medical Inst., Baltimore, USA - Analysis of Bone Marrow Engraftment Following in Utero Bone Marrow Transplantation in a Canine Model.- Part 19: Elaine Lyon, ARUP Laboratories, Salt Lake City, USA - Detection of trisomy 21 using SNP analysis and allele area quantification (Genevieve Pont-Kingdon); Microbiology.- Part 20: C. Drosten, Bernhard-Nocht-Institute of Tropical Medicine, Hamburg, Germany - Rapid Detection