The DNA Replication-Repair Interface

Replication-Coupled Repair, Volume 661 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including  the Repair of replication-born DNA breaks by sister chromatid recombination, High resolution and high throughput DNA cyclization measurements to interrogate DNA bendability, A programmable detection method for genomic signatures: from disease diagnosis to genome editing, Characterization of the telomerase modulating activities of yeast DNA helicases, Eukaryotic DNA replication with purified budding yeast proteins, Single molecule studies of yeast Rad51 paralogs, Light activation and deactivation of Cas9 for DNA repair studies, and more.

Other chapters explore MIDAS: Direct sequencing to map mitotic DNA synthesis and common fragile sites at high precision, Studying the DNA damage response in embryonic systems, GLASS-ChIP to map Mre11 cleavage sites in the human genome, New chemical biology approaches to trap reaction intermediates in living cells, Single-molecule imaging approaches for monitoring replication fork conflicts at genomic DNA G4 structures and R-loops in human cells, Monitoring the replication of structured DNA through heritable epigenetic change, Visualizing replication fork encounters with DNA interstrand crosslinks, and much more.

Preface

1. Eukaryotic DNA replication with purified budding yeast proteins

2. Monitoring the replication of structured DNA through heritable epigenetic change

3. Visualizing replication fork encounters with DNA interstrand crosslinks

4. Single-molecule imaging of replication fork conflicts at genomic DNA G4 structures in human cells

5. Studying the DNA damage response in embryonic systems

6. Analysis of repair of replication-born double-strand breaks by sister chromatid recombination in yeast

7. Determining the kinetics of break-induced replication (BIR) by the assay for monitoring BIR elongation rate (AMBER)

8. Genomic mapping of DNA reaction intermediates in living cells with engineered DNA structure-trap proteins

9. A mapping platform for mitotic crossover by single-cell multi-omics

10. Characterization of DNA-PK-bound end fragments using GLASS-ChIP

11. Light activation and deactivation of Cas9 for DNA repair studies

12. A Detection method for the capture of genomic signatures: from disease diagnosis to genome editing

13. A method to sequence genomic sites of mitotic DNA synthesis in mammalian cells

14. Loop-seq: a high-throughput technique to measure the mesoscale mechanical properties of DNA

15. Characterization of the telomerase modulating activities of yeast DNA helicases

16. Single-molecule studies of yeast Rad51 paralogs

17. Synthesis and polymerase bypass studies of DNA-peptide and DNA-protein conjugates

18. Chemistry and structural screen integrated efficiently for structure-based inhibitors to nucleic acid enzymes targeting the DNA repair-replication interface and SARS CoV-2

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in Methods in Enzymology series
• Includes the latest information on replication-coupled repair