Description
Restriction Endonucleases, Softcover reprint of the original 1st ed. 2004
Coll. Nucleic Acids and Molecular Biology, Vol. 14
Coordinator: Pingoud Alfred
Language: FrenchSubjects for Restriction Endonucleases:
Keywords
ATP; DNA; DNA-Erkennung; DNA-Spaltung; Restriktionsenzym; bacteria; base; enzyme; enzymes; evolution; genetics; nucleic acid; protein; protein engineering; replication
Publication date: 09-2012
443 p. · 15.5x23.5 cm · Paperback
Publication date: 02-2004
443 p. · 15.5x23.5 cm · Hardback
Description
/li>Contents
/li>Comment
/li>
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
These books may interest you
Artificial Nucleases 316.49 €