Salamanders in Regeneration Research, 2015
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1290

This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.

Part I: Salamanders

1. Variation in Salamanders: An Essay on Genomes, Development, and Evolution

Jeremy P Brockes

 2. Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research

Hans-Georg Simon and Shannon Odelberg

3. Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl

Johanna E. Farkas and James R. Monaghan

 4. Husbandry of Spanish Ribbed Newts (Pleurodeles waltl)

Alberto Joven, Matthew Kirkham, and András Simon

 5. Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies

Claudia Marcela Arenas G, Andrea Gómez-Molina, and  Jean Paul Delgado

 Part II: Experimental Manipulation in Salamanders

 6. Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH

Nobuyasu. Maki

 7. Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation

Matthew Kirkham and Alberto Joven

 8. The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration

Tetsuya Endo, David M. Gardiner, Aki Makanae, and Akira Satoh

9. High-efficiency Electroporation of the Spinal Cord in Larval Axolotl

Aida Rodrigo-Albors and Elly M. Tanaka

 10. Pseudotyped Retroviruses for Infecting Axolotl

Tzu-Hsing Kuo and Jessica L. Whited

11. Thyroxine Induced Metamorphosis in the Axolotl (Ambystoma mexicanum)

Peggy S. Coots and Ashley W. Seifert

 12. Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos

Anoop Kumar and Jean Paul Delgado

 13. In vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration

Jami R. Erickson and Karen Echeverri

Part III: Salamander Cells in Culture

14. Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas

Patrizia Ferretti and Anoop Kumar

 15. Culture and Transfection of Axolotl Cells

Jean-François Denis, Fadi Sader, Patrizia  Ferretti, and Stéphane Roy

 16. Isolation and Culture of Neurospheres from the Adult Newt Brain

L Shahul Hameed, András Simon

 17. Methods for Axolotl Blood Collection, Intravenous Injection and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb

Ryan J. Debuque and James W. Godwin

 18. Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture

Hans-Georg Simon and Shannon Odelberg

 19. Long Term Organ Cultures of Newt Hearts

Tanja Piatkowski and Thomas Braun

Ruth R Taylor

 Part IV Transgenesis in Salamanders

21. Transgenesis in Axolotl (Ambystoma mexicanum)

Shahryar Khattak and Elly M Tanaka

 22. Generating and Identifying Axolotls with Targeted Mutations using Cas9 RNA‐guided Nuclease

G Parker Flowers and Craig M Crews

 23. Gene Manipulation for Regenerative Studies using the Iberian Ribbed Newt, Pleurodeles waltl

Toshinori Hayashi and Takashi Takeuchi

 Part V: Gene Expression

24. Transcriptomics Using Axolotls

S. Randal Voss, Anthony Athippozhy, and M. Ryan Woodcock

 25. Sal-Site: Research Resources for The Mexican Axolotl

Nour W Al Haj Baddar, M Ryan Woodcock, Shivam Khatri,  D. Kevin Kump, and S. Randal Voss

Mario Looso and Thomas Braun

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras