Skeletal Muscle Development, 1st ed. 2017
Methods in Molecular Biology Series, Vol. 1668

This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>

Part I: Skeletal Muscle Gene Expression

1. Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells

            Kohei Miyata, Masashi Naito, Tomoko Miyata, Sho Mokuda, and Hiroshi Asahara

2. Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells

            Karl Kamhei So, Xianlu Peng, Hao Sun, and Huating Wang

3. Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle

            Tamar E. Sztal, Peter D. Currie, and Robert J. Bryson-Richardson

4. Chick Somite Electroporation for Gene Function Studies

            Muhammad Abu-Elmagd

Part II: Assays for Proliferating Skeletal Muscle Cells

5. Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS

6. Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells

            C. Hai Ly and James G. Ryall

7. Microcontact-Printed Hydrogel Microwell Arrays for Clonal Muscle Stem Cell Cultures

            Victor M. Aguilar and Benjamin D. Cosgrove

8. Isolation, Culture, and Differentiation of Fibro/Adipogenic Progenitors (FAPs) from Skeletal Muscle

9. Human Satellite Cell Isolation and Xenotransplantation

            Steven M. Garcia, Stanley Tamaki, Xiaoti Xu, and Jason H. Pomerantz

Part III: Assessment of Myogenic Differentiation

10. Application of Split-GFP Reassembly Assay to Study Myogenesis and MyofusionIn Vitro

            Manami Kodaka, Xiaoyin Xu, Zeyu Yang, Junichi Maruyama, and Yutaka Hata

11. Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells

            Toshifumi Asano, Toru Ishizuka, and Hiromu Yawo

12. Fabrication of Micromolded Gelatin Hydrogels for Long-Term Culture of Aligned Skeletal Myotubes

            Gio C. Suh, Archana Bettadapur, Jeffrey W. Santoso, and Megan L. McCain

Part IV: Assessing Skeletal Muscle Development In Vivo

13. Quantification of Embryonic Myofiber Development by Immunofluorescence

            Harika Nagandla and M. David Stewart

14. How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation

Part V: Muscle Repair Assays

15. Membrane Repair Assay for Human Skeletal Muscle Cells

            Romain Carmeille, Coralie Croissant, Flora Bouvet, and Anthony Bouter

16. CryoinjuryModel for Tissue Injury and Repair in Bioengineered Human Striated Muscle

Includes cutting-edge methods and protocols for the study of skeletal muscle cell development

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts