Testicular Germ Cell Tumors, 1st ed. 2021
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2195

Language: English

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Testicular Germ Cell Tumors
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278 p. · 17.8x25.4 cm · Paperback

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Testicular Germ Cell Tumors
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This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field. 


Preface…
Table of Contents…
Contributing Authors…

1. Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research
Payal Kapur and Dinesh Rakheja

2. Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men
Franto Francis and Ming Zhou

3. Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection
Liang Cheng, Steven A. Mann, Antonio Lopez-Beltran, Michal Chovanec, Matteo Santoni, Mingsheng Wang, Costantine Albany, Nabil Adra, Darrell D. Davidson, Alessia Cimadamore, Rodolfo Montironi, and Shaobo Zhang

4. Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors
Liang Cheng, Darrell D. Davidson, Rodolfo Montironi, Mingsheng Wang, Antonio Lopez-Beltran, Timothy A. Masterson, Costantine Albany, and Shaobo Zhang

5. Germ Cell Tumor Cell Culture Techniques 
John Lafin, James F. Amatruda, and Aditya Bagrodia

6. Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops
Margaretha A. Skowron, Meike M. Watolla, and Daniel Nettersheim

7. Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9
Sina Jostes, Daniel Nettersheim, Simon Schneider, and Hubert Schorle

8. Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines
Margaretha A. Skowron, Michèle J. Hoffmann, Meike M. Watolla, and Daniel Nettersheim

9. Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines
Francesca Cavallo, Cinzia Caggiano, Maria Jasin, and Marco Barchi

10. Production and Analysis of Human Primordial Germ Cell-Like Cells
Shino Mitsunaga, Keiko Shioda, Jacob H. Hanna, Kurt J. Isselbacher, and Toshi Shioda

11. A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors
Amy M. Lyndaker, Timothy M. Pierpont, Amanda R. Loehr, and Robert S. Weiss

12. Targeted Methylation Analyses: From Bisulfite Treatment to Quantification 
João Lobo, Ad J. M. Gillis, and Leendert H. J. Looijenga 

13. Integrated Analysis of Germ Cell Tumors
Alok Tewari and Eliezer Van Allen

14. Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors
Anthony J. Hooten, Erica Langer, and Jenny N. Poynter

15. A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring
Matthew J. Murray, Cinzia G. Scarpini, and Nicholas Coleman

16. Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors 
Paulina Nastały, Friedemann Honecker, Klaus Pantel, and Sabine Riethdorf

17. Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors
Bo Ci, Shin-Yi Lin, Bo Yao, Danni Luo, Lin Xu, Mark Krailo, Matthew Murray, James F. Amatruda, A. Lindsay Frazier, and Yang Xie

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts