Description
The Mitotic Spindle, Softcover reprint of the original 1st ed. 2016
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1413
Language: EnglishSubject for The Mitotic Spindle:
Support: Print on demand
Description
/li>Contents
/li>Comment
/li>
This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field.
Part I Methods Focused on Cytology
1: Using Fluorescence Microscopy to Study Mitosis|Sai K. Balchand, Barbara J. Mann, and Patricia Wadsworth
2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation
Bin He and Daniela Cimini
Part II Methods Focused on Microtubules and the Mitotic Spindle
3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts
Aaron C. Groen and Timothy J. Mitchison
4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro
Marija Zanic
5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles
Nicolas Lecland and Jens Lüders
6:Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy
Matthew King and Sabine Petry
7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size
Matthew C. Good
Part III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface
8:In Vitro Kinetochore Assembly
Matthew D. D. Miell and Aaron F. Straight
9: Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes
Tatsuya Nishino and Tatsuo Fukagawa
10:Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells
Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca
11: Studying Kinetochores In Vivo Using FLIM-FRET
Tae Yeon Yoo and Daniel J. Needleman
Section IV Methods Focused on the Spindle Pole
12: Purification of Fluorescently LabeledSaccharomyces Cerevisiae Spindle Pole Bodies
Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis
13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion
Rajesh Kumar Soni and Meng-Fu Bryan Tsou
14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation
Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo
Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins
15: Analyzing Spindle Positioning Dynamics in Cultured Cells
Tomomi Kiyomitsu
16:Quantification of Mitotic Chromosome Alignment
Cindy Fonseca and Jason Stumpff
17: Imaging Mitosis in the Moss Physcomitrella Patens
Moé Yamada, Tomohiro Miki, and Gohta Goshima
18: Small Molecule Approach to Study the Function of Mitotic Kinesins
Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer
Part VI Novel Approaches to Study Spindle Function and Regulation
19: Identification and Characterization of Mitotic Spindle-Localized Transcripts
Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower
20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization
Edward R. Ballister and Michael A. Lampson
21: Studying Kinetochore Kinases
Adrian T. Saurin and Geert J.P.L. Kops
22: Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics
Mathew J.K. Jones and Prasad V. Jallepalli
Part VII The Mitotic Spindle and Cancer
23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer
Mijung Kwon
24: Generation and Purification of Tetraploid Cells
Elizabeth M. Shenk and Neil J. Ganem
25: Anti-Microtubule Drugs
Stefan Florian and Timothy J. Mitchison
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
These books may interest you
MitosisMethods and Protocols 110.76 €
MitosisMethods and Protocols 105.49 €