Description
The Nucleus (3rd Ed., 3rd ed. 2020)
Methods in Molecular Biology Series, Vol. 2175
Language: EnglishSubject for The Nucleus :
Publication date: 07-2021
220 p. · 17.8x25.4 cm · Paperback
Publication date: 07-2020
Support: Print on demand
Description
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1. Live-Cell Imaging and Analysis of Nuclear Body Mobility
Dmitry V. Sorokin, Eugene A. Arifulin, Yegor S. Vassetzky, and Eugene V. Sheval
2. Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell
David Anchel and Reagan W. Ching
3. Visualizing Chromatin Modifications in Isolated Nuclei
Yuan Li, Zachary Klase, and Luca Sardo
4. Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture
Alexey I. Chizhik, Anna M. Chizhik, Daja Ruhlandt, Janine Pfaff, Narain Karedla, Ingo Gregor, Ralph H. Kehlenbach, and Jörg Enderlein
5. Studying Proton Gradients Across the Nuclear Envelope
Raul Martínez-Zaguilán and Souad R. Sennoune
6. COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes
Michael Hausmann, Jin-Ho Lee, Aaron Sievers, Matthias Krufczik, and Georg Hildenbrand
7. Genome-Wide Mapping of UV-Induced DNA Damage with CPD-Seq
Peng Mao and John J. Wyrick
8. AP-Seq: A Method to Measure Apurinic Sites and Small Base Adducts Genome-Wide
Anna R. Poetsch
9. Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID
Enes Ugur, Michael D. Bartoschek, and Heinrich Leonhardt
10. Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions
David Umlauf, Michal Sobecki, and Laure Crabbe
11. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres
Šárka Schořová, Jiří Fajkus, and Petra Procházková Schrumpfová
12. Macromolecular Crowding Measurements with Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells
Sara N. Mouton, Liesbeth M. Veenhoff, and Arnold J. Boersma
13. Analysis of a Nuclear Intrinsically Disordered Proteome
Bozena Skupien-Rabian, Urszula Jankowska, and Sylwia Kedracka-Krok
14. Timing of Cytosine Methylation on Newly-Synthesized RNA by Electron Microscopy
Lorena Zannino, Stella Siciliani, and Marco Biggiogera
15. The Nucleus of Intestinal Cells of the Bacterivore Nematode Caenorhabditis elegans as a Sensitive Sensor of Environmental Pollutants
Annette Piechulek, Lutz Berwanger, Peter Hemmerich, and Anna von Mikecz
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts