The TNF Superfamily (2^nd Ed., 2nd ed. 2021)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2248

This second edition focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Chapters detail protocols on the signaling process of TNF family members, technical examples to investigating the role of TNF family members in physiopathologies, modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, methodologies for various assays of TNF family members and the production of recombinant molecules.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, The TNF Superfamily: Methods and Applications, Second Edition serves to aid researchers investigating this key family of proteins.

1. Signaling in TNFSF15-mediated Suppression of VEGF Production in Endothelial Cells

Huanyu Zhao and Qiangzhe Zhang

2. Sensitization of Airway Epithelial Cells to Toxin-Induced Death by TNF Superfamily Cytokines

Claire Reynolds-Peterson, Dylan J Ehrbar, Susanne M McHale, Timothy J. LaRocca, and Nicholas J Mantis

3. Inhibition of Chondroitin Sulfate Proteoglycans by APRIL

Mashal Claude Ahmed and Bertrand Huard

4. Induction of Antigen-independent Proliferation of Regulatory T-cells by TNF superfamily ligands OX40L and GITRL

Prabhakaran Kumar, Zarema H Arbieva, Mark Maienschein-Cline, Balaji B Ganesh, Suresh Ramasamy, and Bellur S Prabhakar

5. Transition from TNF-induced Inflammation to Death Signaling

6. Analysis of FcgR-Dependent Agonism Of Antibodies Specific for Receptors of the Tumor Necrosis Factor (TNF) Receptor Superfamily (TNFRSF)

7. Generation and Evaluation of Bispecific anti-TNF Antibodies Based on Single-Chain V_HH Domains

M.A. Nosenko, K-S.N. Atretkhany, V.V. Mokhonov, S.A. Chuvpilo, D.V. Yanvarev, M.S. Drutskaya, S.V. Tillib, and S.A. Nedospasov

Wenyu Fu, Aubryanna Hettinghouse, and Chuan-ju Liu

9. Fluorescence Based TNFR1 Biosensor for Monitoring Receptor Structural and Conformational Dynamics and Discovery of Small Molecule Modulators

Chih Hung Lo, Tory M. Schaaf, David D. Thomas, and Jonathan N. Sachs

10. Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development

Ting-Hsuan Chen, Jo-Yu Hong, Chia-Chyi Liu, Chung-Chu Chen, Jia-Tsrong Jan, and Suh-Chin Wu

11. Experimental Methods for the Immunological Characterization of Paradoxical Psoriasis Reactions Induced by TNF-α Biologics

Martina Morelli, Claudia Scarponi, Stefania Madonna, and Cristina Albanesi

Sonia Schuepbach-Mallepell, Christine Kowalczyk-Quintas, Angela Dick, Mahya Eslami, Michele Vigolo, Denis J. Headon, Michael Cheeseman, Holm Schneider, and Pascal Schneider

13. Analysis of Ligand-Receptor Interactions Using Bioluminescent TNF Superfamily (TNFSF) Ligand Fusion Proteins

Kirstin Kucka, Juliane Medler, and Harald Wajant

14. Monitoring Atsttrin-mediated Inhibition of TNFα/NF-κβ Activation through in vivo Bioluminescence Imaging

Aubryanna Hettinghouse, Wenyu Fu, and Chuan-ju Liu

15. The use of Murine Infection Models to Investigate the Protective Role of TNF in Central Nervous System Tuberculosis

Nai-Jen Hsu and Muazzam Jacobs

16. Identification of CD137- and CD137L-expressing Cells in EL-4 Tumor

Sang W. Kang, Hong R. Cho,and Byungsuk Kwon

17. Expression Profiling of Tumor Necrosis Factor Superfamily Ligands mRNA in
Healthy and Injured Murine Kidneys

Bhawna Tomar and Shrikant R. Mulay

18Analysis of Lymphotoxin Alpha Expression in Human Retina and Generation of Expression Vectors to Functional Characterization of Polymorphisms in the Tumor Necrosis Factor Locus

Ricardo Usategui-Martín, Irene Rodriguez-Hernández, Rogelio González-Sarmiento, Eva M. Sobas, Jose Carlos Pastor Jimeno, and Salvador Pastor Idoate

19. Detection of TNF-α Protein in Extracellular Vesicles Derived from Tumor Cells by Western Blotting

 Tandressa Souza Berguetti, Raquel Ciuvalschi Maia, and Paloma Silva de Souza

20. Expression of TNFRs by B and T lymphocytes in Tumor-Draining Lymph Nodes

Atri Ghods, Abbas Ghaderi, and Fereshteh Mehdipour

Kirti Hira and Sajeli A Begum

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts