Description
Western Blotting Guru
Authors: Najafov Ayaz, Hoxhaj Gerta
Language: EnglishSubjects for Western Blotting Guru:
Keywords
Air bubbles; Band smear; Bubbles; Data retrieval; Data storage; Digitization; ECL; Electrodes; Faint signal; Fresh buffers; Gel electrophoresis; Gel running; George Stark; Glass plates; Harry Towbin; High background; Immunoblotting; Loading replicates; Membrane stripping; Metadata; Multiple antibodies trick; Multiple bands; Nitrocellulose; No bands detected; Nonphospho peptides; Nonreducing PAGE; Overlay assays; PVDF; Phos-Tag and dot blotting; Phospho-specific antibodies; Polyacrylamide gels; Ponceau S; Premix gels; Primary antibody; Quantification; Quantitative Western blotting; Quick stripping; SDS-PAGE; Sample viscosity; Scanning; Searchable; Secondary antibody; Smiling effect; Transfer; TrueBlot secondary antibody; W Neal Burnette; Western blotting; Wet transfer versus semidry
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Description
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Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types.
1. Introduction2. Procedure3. Good Practices4. Troubleshooting5. Tips and Tricks6. Special Cases
researchers and students in biochemistry, molecular biology, cell biology, immunology
Gerta was born in Fier, Albania. She obtained her BSc degrees in Molecular Biology and Genetics, as well as a second major in Chemistry from Bosphorus University, Istanbul, Turkey. Gerta pursued her PhD in Biochemistry at MRC Protein Phosphorylation Unit at Dundee University, Scotland, UK in Prof. Carol MacKintosh’s lab. Gerta is currently a postdoctoral fellow in Prof. Brendan Mannings’s lab at Harvard T.H. Chan School of Public Health, Department of Genetics and Complex Diseases.
- Provides a benchtop reference that every molecular biologists will use to design, optimize, troubleshoot and analyze their immunoblotting experiments
- Contains unique good practices and tips that are indispensable for the beginner and expert alike
- Features special cases with applications of immunoblotting optimization
- Includes detailed appendices with tables, figures and key protocols
- Provides troubleshooting tips for various types of modifications of standard protocols
- Organized as a systematic, concentrated resource to save time when addressing an immunoblotting problem