Wnt Signaling, 1st ed. 2016
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1481

Language: English

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Wnt Signaling
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Wnt Signaling
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Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management.

1. Visualizing Wnt Palmiteoylation in Single Cells

Xinxin Gao and Rami N. Hannoush

2. Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction

Rubina Tuladhar, Nageswari Yarravarapu, and Lawrence Lum

3. Biochemical Methods to Analyze Wnt Protein Secretion

Kathrin Glaeser, Michael Boutros, and Julia Christina Gross

4. Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum

Xinjun Zhang and Xi He

5. Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway

Xiaomo Jiang and Feng Cong

6. A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells

Annette S. Flozak, Anna P. Lam, and Cara J. Gottardi

7. Wnt-Dependent Control of Cell Polarity in Cultured Cells

Kristin B. Runkle and Eric S. Witze

8. The Use of Chick Embryos to Study Wnt Signaling/Gradients

Lisa M. Galli, Tiffany Barnes, and Laura W. Burrus

9. Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors

Nicola Facchinello, Marco Schiavone, Andrea Vettori, Francesco Argenton, and Natascia Tiso

10. Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo

Jesung Moon and James F. Amatruda

11. Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts

Annastasia Simone Hyde, Brian I. Hang, and Ethan Lee

12. Delivery of the Porcupine Inhibitor WNT974 in Mice

Li-shu Zhang and Lawrence Lum

13. Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis

Zhendong A. Zhong, Nicole J. Ethen, and Bart O. Williams

14. Monitoring Wnt/b-Catenin Signaling in Skin

Amy Ku, Qi Miao, and Hoang Nguyen

<15. The Generation of Organoids for Studying Wnt Signaling

Jarno Drost, Benedetta Artegiani, and Hans Clevers

16. Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells

Ian Huggins, David Brafman, and Karl Willert

17. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions

Xiaoping Bao, Xiaojun Lian, and Sean P. Palecek


Includes cutting-edge methods and protocols for the study of Wnt signaling

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts