Yeast Protocols (4^th Ed., 4th ed. 2021)
Methods in Molecular Biology Series, Vol. 2196

This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.

1. Use of Yeast Plasmids - Transformation and Inheritance Assays

Anastasiia Mereshchuk, Joyce S.K. Chew, and Melanie J. Dobson

2. Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae

Seiji Tanaka

3. CRISPR Nickase-Mediated Base Editing in Yeast

Kouichi Kuroda and Mitsuyoshi Ueda

Xuelei Tian, Wenqing Zhang, and Wei Xiao

5. Study Essential Gene Functions by Plasmid Shuffling

Li Fan and Wei Xiao

Qian Wang, Yu V. Fu, and Wei Xiao

7. High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae

Fanli Zeng and David G. Quintana

Xiuling Cao, Xuejiao Jin, and Beidong Liu

9. Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe

Ming He, Xu Chi, and Jie Ren

10. Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map

Zhiyun Xu, Jianxun Feng, and Qing Li

11. Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly

Yixuan Zhu and Limin Cao

12. Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE

Zhouqing Luo, Shuangying Jiang, and Junbiao Dai

13. Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering

Shuangying Jiang, Zhouqing Luo, and Junbiao Dai

14. A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9

Nicolas Agier, Aubin Fleiss, Stéphane Delmas^, and Gilles Fischer

15. Yeast Nucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA

Yong Wang and Yu V. Fu

16. Assays for Autophagy III: Observing Dynamic Protein Trafficking

Jing Zhu and Zhiping Xie

17. Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification

Xuejiao Jin, Xiuling Cao, and Beidong Liu

18. High-Throughput Rapid Yeast Chronological Lifespan Assay

Abd-Al-Wahab A. Khawaja, Zach R. Belak, Christopher H. Eskiw, and Troy A. A. Harkness

19. Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast

Shanshan Zhang, Hui Li, and Ji-Long Liu

20. Monitoring 5’-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis

Haoyang Peng, Simin Zhang, and Xuefeng Chen

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts