Animal Influenza Virus (3^rd Ed., 3rd ed. 2020) Methods and Protocols Methods in Molecular Biology Series, Vol. 2123

This third edition aims to provide new and updated methods on animal influenza viruses as well as more advanced protocols that will guide the reader in designing research. Chapters detail influenza in peridomestic animals, marine mammals, savian influenza, swine influenza, equine influenza, hemagglutination,  genome sequencing, and influenza in other mammals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Animal Influenza Virus: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

Part I: General

1. Hemagglutination Assay for Influenza Virus

Mary Lea Killian

2. Hemagglutination Inhibition Assay

Erica Spackman and Ioannis Sitaras

3. Immunohistochemical Staining of Influenza Virus in Tissues

Mary J. Pantin-Jackwood

4. Plasmid-based Reverse Genetics of Influenza A virus

Daniel R. Perez, Brittany Seibert, Lucas Ferreri, Chang-Won Lee,and Daniela Rajao

5. Antigenic Cartography: Overview and Current Developments

6. Complete Genome Sequencing of Influenza A viruses using Next-G\generation Sequencing

Dong-Hun Lee

Part II: Avian Influenza

7. A Brief Introduction to Avian Influenza Virus

Erica Spackman

Rebecca L. Poulson and Justin D. Brown

9. Avian Influenza Virus Sample Types, Collection, and Handling

Mary Lea Killian

10. Avian Influenza Virus RNA Extraction

Erica Spackman and Scott A. Lee

11. Avian Influenza Virus Detection and Quantitation by Real-time RT-PCR

Erica Spackman

12. Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs

Erica Spackman and Mary Lea Killian

13. Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus

Terra A. Jenson 

Erica Spackman and Mary Lea Killian

15. Conducting Influenza Virus Pathogenesis Studies in Avian Species

Carol Cardona, David A. Halvorson, Justin Brown, and Mary Pantin-Jackwood

16. Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry

David E. Swayne

17. Techniques for the Measurement of Cell Mediated Immune Responses to Avian Influenza Virus

Part III: Swine Influenza

18. A Brief Introduction to Influenza A Virus in Swine

Amy L. Vincent, Tavis K. Anderson, and Kelly M. Lager

19.Specimen Types, Collection, and Transport for Influenza A Viruses of Swine

Marie Culhane, Jorge Garrido-Mantilla, and Montserrat Torremorell

Jianqiang Zhangand Phillip C. Gauger

21. RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-time RT-PCR

22. Enzyme-linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype SpecificIgG and IgA Whole Virus Antibody to Influenza A virus in Swine

Phillip C. Gauger and Amy L. Vincent

23. Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine

Phillip C. Gauger and  Amy L. Vincent

Bryan S. Kaplan and Amy L. Vincent

25. In vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza

IV: Equine Influenza

26. A Brief Introduction to Equine Influenza and Equine Influenza Viruses

27. Equine Influenza Diagnosis: Sample Collection and Transport

Thomas M. Chambers and Stephanie E. Reedy

28. RNA Extraction from Equine Samples for Equine Influenza Virus

Udeni B. R. Balasuriya

29. Type A Influenza Virus Detection from Horses by Real-Time RT-qPCR and Insulated Isothermal RT-PCR 

Udeni B. R. Balasuriya

30. Equine Influenza Culture Methods

Thomas M. Chambers and Stephanie E. Reedy

31. Equine Influenza Serological Methods

Thomas M. Chambers and Stephanie E. Reedy

32. Influenza A Viruses in Peridomestic Mammals

J. Jeffrey Root   and Susan A. Shriner

33. A Brief Introduction to Influenza A Virus in Marine Mammals

Jonathan A. Runstadler and Wendy Puryear

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts