Biochemistry in the Lab A Manual for Undergraduates
Auteur : Lasseter Benjamin F.
Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps.
Key Features:
- Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience
- Includes chemical biology as its foundation, teaching readers experimental methods specific to the field
- Provides instructor experiments that are easy to prepare and execute, at comparatively low cost
- Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry
- Is written by an expert in the field
This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.
Author Bio
Chapter 1 Buffers
Chapter 2 Assays
Chapter 3 Protein Concentration
Chapter 4 ELISA
Chapter 5 Salting Out Proteins and Other Biomolecules
Chapter 6 A Discussion of Isoelectric Point and Effective Charge
Chapter 7 Column Chromatography
Chapter 8 Michaelis–Menten Kinetics
Chapter 9 Protein Purification
Chapter 10 Polyacrylamide Gels
Chapter 11 In Silico Biochemistry: The Evolution of Globins
Chapter 12 Growing Crystals of Hemoglobin
Chapter 13 Enzyme Inhibition
Chapter 14 Multisubstrate Kinetics
Chapter 15 Fluorescence and Denaturation
Chapter 16 Fluorescence Studies of Ligand Binding
Chapter 17 DNA Restriction Digests
Chapter 18 Western Blotting
Index
Dr. Benjamin F. Lasseter earned his Ph.D. in Biochemistry from Texas A&M University in 2003. He briefly assisted in the production of history textbooks for the Catholic Schools Textbook Project before teaching at a high school in Louisiana. He moved on to Christopher Newport University in 2011, where he teaches the biochemistry labs. He mixes analytical chemistry with pure biology to study environmental impacts of metal pollution.
Date de parution : 10-2019
15.6x23.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 14 jours).
Prix indicatif 209,69 €
Ajouter au panierDate de parution : 10-2019
15.6x23.4 cm
Thèmes de Biochemistry in the Lab :
Mots-clés :
TRIS Glycine Buffer; Sodium Phosphate Monobasic; Protein chemistry; protein gel techniques; protein purification; Lineweaver Burke Plot; Biochemistry laboratory techniques; Michaelis Constant; Enzymology; crystal growth; unfolding studies; and fluorescence; Ammonium Sulfate; Modern biochemistry laboratory; Tae Buffer; modern experimental biochemistry; Negative Cooperativity; instructor experiments; EA Complex; biochemistry laboratory teaching; Ion Exchange Chromatography; protein purification; Vice Versa; Cap; PVDF Membrane; Bovine Catalase; Tris Acetate EDTA Buffer; DNA Agarose Gel; Sodium Phosphate Dibasic; SDS Page Analysis; PDB Id; Bradford Reagent; Toyopearl HW-75; BSA Standard; Native Page; Enzyme Linked ImmunoabSorption Assay; DNA Mutagenesis; Orbital Energy Level
