Cancer Cytogenetics, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1541

This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting. The updated reviews on chromosomal abnormalities in hematological malignancies are excellent guides for cytogenetics data interpretations and specific malignant diseases correlation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Cancer Cytogenetics: Methods and Protocols is a valuable resource for the novice in cytogenetics because it provides helpful guiding protocols, but it?s also great for those who are already engaged in the field and are looking for some technical hints.

Preface…
Table of Contents…
Contributing Authors…

1. Cancer Cytogenetics: An Introduction

Thomas S.K. Wan

2. Chromosome Preparation for Myeloid Malignancies

Eleanor K.C. Hui, Thomas S.K. Wan, and Margaret H.L. Ng

3. Chromosome Preparation for Acute Lymphoblastic Leukemia

4. Chromosome Preparation for Chronic Lymphoid Malignancies

5. Cytogenetic Harvesting of Cancer Cells and Cell Lines

Roderick A.F. MacLeod, Maren E. Kaufmann, and Hans G. Drexler

6. Chromosome Bandings

Huifang Huang and Jiadi Chen

7. Chromosome Recognition

Thomas S.K. Wan, Eleanor K.C. Hui, and Margaret H.L. Ng

8. Application of Fluorescence In Situ Hybridization Technology in Malignancies

Montakarn Tansatit

9. Fluorescence In Situ Hybridization Probe Preparation

Doron Tolomeo, Roscoe R. Stanyon, and Mariano Rocchi

10. Fluorescence In Situ Hybridization Probe Validation for Clinical Use

Jun Gu, Janice L. Smith, and Patricia K. Dowling

11. Fluorescence In Situ Hybridization on Tissue Sections

Alvin S.T. Lim and Tse Hui Lim

13. Quantitate Fluorescence In Situ Hybridization (QFISH)

Ivan Iourov

14. High Resolution of Fiber-Fluorescence In Situ Hybridization (Fiber-FISH)

Christine J. Ye and Henry H. Heng

15. Array-Based Comparative Genomic Hybridization (aCGH)

16. Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND)

Thomas Liehr, Moneeb A.K. Othman, and Katharina Rittscher

17. Cytogenetics for Biological Dosimetry

Michelle Ricoul, Tamizh Gnana-Sekaran, Laure Piqueret-Stephan, and Laure Sabatier

18. Recurrent Cytogenetic Abnormalities in Myelodysplastic Syndromes

Meaghan Wall

John J. Yang, Tae Sung Park, and Thomas S.K. Wan

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras