Handbook of Biochemistry and Molecular Biology (5^th Ed.)

Edited by renowned protein scientist and bestselling author Roger L. Lundblad, with the assistance of Fiona M. Macdonald of CRC Press, this fifth edition of the Handbook of Biochemistry and Molecular Biology gathers a wealth of information not easily obtained, including information not found on the web.

Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents. An entirely new section on Chemical Biology and Drug Design gathers data on amino acid antagonists, click chemistry, plus glossaries for computational drug design and medicinal chemistry. Each table is exhaustively referenced, giving the user a quick entry point into the primary literature.

New tables for this edition:

• Chromatographic methods and solvents





• Protein spectroscopy





• Partial volumes of amino acids





• Matrix Metalloproteinases





• Gene Editing





• Click Chemistry

SECTION I: AMINO ACIDS, PEPTIDES, AND PROTEINS

Properties of Amino Acids

Data on the Naturally Occurring Amino Acids

Coefficients of Solubility Equations of Certain Amino Acids in Water

Heat Capacities, Absolute Entropies, and Entropies of Formation of Amino Acids and Related Compounds

Heat of Combustion, Enthalpy, and Free Energy of Formation of Amino Acids and Related Compounds

Solubilities of Amino Acids in Water at Various Temperatures

Heats of Solution of Amino Acids in Aqueous Solution at 25°C

Free Energies of Solution and Standard Free Energy of Formation of Amino Acids in Aqueous Solution at 25°C

Far Ultraviolet Absorption Spectra of Amino Acids

UV Absorption Characteristics of N-Acetyl Methyl Esters of the Aromatic Amino Acids, Cystine, and of N-Acetylcysteine

Numerical Values of the Absorbances of the Aromatic Amino Acids in Acid, Neutral, and Alkaline Solutions

Hydrophobicities of Amino Acids and Proteins

Partial Volumes of Amino Acids

Deamidation of Asparagine

Michael Reactions of Proteins

Molecular Weights of Proteins and Some Other Materials Including Sedimentation, Diffusion, and Frictional Coefficients and Partial Specific Volumes

Molar Absorptivity and A1cm 1% Values for Proteins at Selected Wavelengths of the Ultraviolet and Visible Region

Temperature Coefficients of Apparent Partial Specific Volumes of Proteins Expressed in ml/g/deg (dV/dT)

Protein pK Values

Intrinsic Viscosity of Proteins

Infrared Spectra of Proteins

Volume Changes for Some Macromolecule Association Reactions

Assay of Solution Protein Concentration

Spectrophotometric Determination of Protein Concentration in the Short-Wavelength Ultraviolet

SECTION II: ENZYMES

Turnover of Enzymes in Tissues

Molecular Turnover of Cytochromes in Animals

Matrix Metalloproteinases

Proteases in the Interstitial Space

Enzymes in Synthetic OrganicChemistry

Therapeutic Enzymes

Protease Inhibitors and Protease Inhibitor Cocktails

SECTION III: LIPIDOMICS AND LIPIDOMES

Evolution of the Classification and Nomenclature of Lipids

A Brief Glossary for Lipidomics

A Comprehensive Classification System for Lipids

Properties of Fatty Acids and Their Methyl Esters

Densities, Specific Volumes, and Temperature Coefficients of Fatty Acids from C8 to C12

Composition and Properties of Common Oils and Fats

Spectral Properties of Fatty Acids

IR Active Bands of Phosphatidylethanolamines (PE)

Concentration of Some Fatty Acids in Human Blood, Blood Plasma or Blood Serum

Some Unusual Fatty Acid Structures

SECTION IV: VITAMINS AND COE NZYMES

Properties of Vitamins

Biological Characteristics of Vitamins

Vitamers

Vitamin Names Discarded

SECTION V: NUCLEIC ACIDS

Some Definitions for Nucleic Acids

UV Spectral Characteristics and Acidic Dissociation Constants of 280 Alkyl Bases, Nucleosides, and Nucleotides

Ultraviolet Absorbance of Oligonucleotides Containing 2′-O-Methylpentose Residues

Spectrophotometric Constants of Ribonucleotides

Purines, Pyrimidines, Nucleosides, and Nucleotides: Physical Constants and Spectral Properties

Chemical Modification of Nucleic Acids

Polymerase Chain Reaction

Gene Editing

SECTION VI: GLYCOSCIENCE

Nomenclature of Carbohydrates

Chemical Modification of Carbohydrates

Some Properties of Glycosaminoglycans

Sugar Mimetics

Intrinsic Viscosity of Polysaccharides

SECTION VII: CHEMICAL BIOLOGY AND DRUG DESIGN

Unnatural Amino Acids for Incorporation into Proteins

Structures and Symbols for Synthetic Amino Acids Incorporated into Synthetic Polypeptides

Properties of the α-Keto Acid Analogs of Amino Acids

α,β-Unsaturated Amino Acids

Amino Acid Antagonists

Particle Diameter

Weights of Cells and Cell Constituents

Click Chemistry

A Listing of Log P Values, Water Solubility, and Molecular Weight for Some Selected Chemicals

SECTION VIII: PHYSICAL AND CHEMICAL DATA

IUBMB-IUPAC Joint Commission on Biochemical Nomenclature (JCBN) Recommendations for Nomenclature and Tables in Biochemical Thermodynamics

Standard Transformed Gibbs Energies of Formation for Biochemical Reactants

Enthalpy, Entropy, and Free Energy Values for Biochemical Redox Reactions

Oxidation-Reduction Potentials, Absorbance Bands, and Molar Absorbance of Compounds Used in Biochemical Studies

Calorimetric ΔH Values Accompanying Conformational Changes of Macromolecules in Solution

Free Energies of Hydrolysis and Decarboxylation

Deci-Normal Solutions of Oxidation and Reduction Reagents

Guidelines for Potentiometric Measurements in Suspensions Part A — The Suspension Effect

Ionization Constants of Acids and Bases

Guidelines for NMR Measurements for Determination of High and Low pKa Values

Measurement and Interpretation of Electrokinetic Phenomena

Measurement of pH Definition, Standards, and Procedures

General Comments on Buffers

List of Buffers

Bronsted Acidities

Buffer Solutions

Amine Buffers Useful for Biological Research

Preparation of Buffers for Use in Enzyme Studies

Indicators for Volumetric Work and pH Determinations

Acid and Base Indicators

Specific Gravity of Liquids

Properties of Some Solvents

Chemicals Commonly Used in Biochemistry and Molecular Biology and their Properties

Common Detergents Used in Biochemical Research

Some Properties of Detergents and Surfactants Used in Biochemistry and Molecular Biology

Some Biological Stains and Dyes

Mordant Dyes

Metal Chelating Agents

Metallomics: Guidelines for Terminology and Critical Evaluation of Analytical Chemistry Approaches (IUPAC Technical Report)

Water

General Information on Spectroscopy

Plastics

Chemical and Physical Properties of Various Commercial Plastics

Chemical Resistance of Plastics

Generic Source-Based Nomenclature for Polymers

Definitions of Terms Relating to Reactions of Polymers and to Functional Polymeric Materials

Definitions of Terms Related to Polymer Blends, Composites, and Multiphase Polymeric Materials

Terminology for Biorelated Polymers and Applications (IUPAC Recommendations 2012)

Intrinsic Viscosity of Poly(ethylene glycol)

International Union of Pure and Applied Chemistry

Organic Name Reactions Useful in Biochemistry and Molecular Biology

Brief Guide to the Nomenclature of Inorganic Chemistry

Properties of Fluorescent Probes Commonly Used for Membrane Studies

Chromatography

Blotting Technologies

Oxidation of Thiols

Partial Volumes for Some Selected Compounds

Appendix: Abbreviations and Acronyms

Roger L. Lundblad, Ph.D.

Roger L. Lundblad is a native of San Francisco, California. He received his undergraduate education at Pacific Lutheran University and his PhD degree in biochemistry at the University of Washington. After postdoctoral work in the laboratories of Stanford Moore and William Stein at the Rockefeller University, he joined the faculty of the University of North Carolina at Chapel Hill. He joined the Hyland Division of Baxter Healthcare in 1990. Currently Dr. Lundblad is an independent consultant and writer in biotechnology in Chapel Hill, North Carolina. He is an adjunct Professor of Pathology at the University of North Carolina at Chapel Hill and Editor-in-Chief of the Internet Journal of Genomics and Proteomics.

Fiona M. Macdonald, Ph.D., F.R.S.C.

Fiona M. Macdonald received her BSc in chemistry from Durham University, UK. She obtained her PhD in inorganic biochemistry at Birkbeck College, University of London, studying under Peter Sadler. Having spent most of her career in scientific publishing, she is now at Taylor and Francis and is involved in developing chemical information products.