Hemorrhagic Fever Viruses, 1st ed. 2018
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1604

Coordinator: Salvato Maria S.

Language: English

121.31 €

Subject to availability at the publisher.

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Hemorrhagic Fever Viruses
Support: Print on demand

168.79 €

Subject to availability at the publisher.

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Hemorrhagic Fever Viruses
Support: Print on demand
This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection.

Preface

Table of Contents

Contributing Authors

 

Part I Surveillance, Diagnosis and Classification of hemorrhagic fever viruses

 1. Global Spread of Hemorrhagic Fever Viruses: Predicting Pandemics
Jean Paul Gonzalez, Marc Souris, and Willy Valdivia-Granda


2. An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses
Francisco J. Díaz, Luis Paternina, and Juan D. Rodas

3. Preliminary Classification of Novel Hemorrhagic Fever-Causing Viruses Using Sequence-Based Pairwise Sequence Comparison (PASC) Analysis
Yīmíng Bào and Jens H. Kuhn

4. Epidemiological Surveillance of Viral Hemorrhagic Fevers with Emphasis on Clinical Virology
Carolina Montoya-Ruiz and Juan D. Rodas

5. Diagnostics for Lassa Fever: Detecting Host Antibody Responses
Maria S. Salvato, Igor S. Lukashevich, Sandra Medina-Moreno, and Juan Carlos Zapata

6. Sampling Design and Mosquito Trapping for Surveillance of Arboviral Activity
Luis E. Paternina and Juan D. Rodas

7. Epidemiological Surveillance of Rodent-Borne Viruses (Roboviruses)
Juan D. Rodas, Andrés Londoño, and Sergio Solari

 

Part II Structural Studies and Reverse Genetics of Hemorrhagic Fever Viruses


8. Entry Studies of New World Arenaviruses
María Guadalupe Martínez, María Belén Forlenza, Nélida A. Candurra, and Sandra M. Cordo

9. Studies of Lassa Virus Cell Entry
Antonella Pasquato, Antonio Herrador Fernandez, and Stefan Kunz

10. A Cell-Cell Fusion Assay to Assess Arenavirus Envelope Glycoprotein Membrane-Fusion Activity
Joanne York and Jack H. Nunberg

11. Assays to Assess Arenaviral Glycoprotein Function
Junjie Shao, Xiaoying Liu, Yuying Liang, and Hinh Ly

12. Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus
Xiaoxuan Qi, Wenjian Wang, Haohao Dong, Yuying Liang, Changjiang Donga, and Hinh Ly

13. Assays to Demonstrate the Roles of Arenaviral Nucleoproteins (NP) in Viral RNA Synthesis and in Suppressing Type I Interferon
Qinfeng Huang, Junjie Shao, Yuying Liang, and Hinh Ly

14. Intracellular Detection of Viral Transcription and Replication using RNA FISH
Michael E. Lindquist and Connie S. Schmlajohn 

15. Hemorrhagic Fever Virus Budding Studies
Ronald N. Harty

 

Part III In Vivo Models of Hemorrhagic Fever Virus Infection


16. Murine Models for Viral Hemorrhagic Fever
Rosana Gonzalez-Quintial and Roberto Baccala

17. Testing Experimental Therapies in a Guinea Pig Model for Hemorrhagic Fever
Gary Wong, Yuhai Bi, Gary Kobinger, George F. Gao, and Xiangguo Qiu

18. A Primate Model for Viral Hemorrhagic Fever
Maria S. Salvato, Igor S. Lukashevich, Yida Yang, Sandra Medina-Moreno, Mahmoud Djavani, Joseph Bryant, and Juan C. Zapata

19. A Primary Human Liver Cell Culture Model for Hemorrhagic Fever Viruses
Mahmoud Djavani

 

Part IV Immune Assays and Vaccine Production for Hemorrhagic Fever Viruses


20. Protocol for the Production of a Vaccine Against Argentine Hemorrhagic Fever
Ana María Ambrosio, Mauricio Andrés Mariani, Andrea Soledad Maiza, Graciela Susana Gamboa, Sebastián Edgardo Fossa, and Alejando Javier Bottale

21. Detection of Virus-Antibody Immune Complexes in Secondary Dengue Virus Infection
Meng Ling Moi, Tomohiko Takasaki, and Ichiro Kurane

22. Future Approaches to DNA Vaccination Against Hemorrhagic Fever VirusesJohn J. Suschak and Connie S. Schmaljohn

 

Part V Host Responses to Viral Hemorrhagic Fever


23. Identifying Restriction Factors for Hemorrhagic Fever Viruses: Dengue and Junín
Federico Giovannoni, Jose Rafael Peña Cárcamo, María Laura Morell, Sandra Myriam Cordo, and Cybele Carina García

24. Determining the Virus Life-Cycle Stage Blocked by an Antiviral
Claudia S. Sepúlveda, Cybele C. García, and Elsa B. Damonte

25. Retrovirus-Based Surrogate Systems for BSL-2 High-Throughput Screening of Antivirals Targeting BSL-3/4 Hemorrhagic Fever-Causing Viruses
Sheli R. Radoshitzky, Veronica Soloveva, Dima Gharaibeh, Jens H. Kuhn, and Sina Bavari

26. Protocols to Assess Coagulation following In Vitro Infection with Hemorrhagic Fever Viruses
Melissa L. Tursiella, Shannon L. Taylor, and Connie S. Schmaljohn

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras