Kidney Research, Softcover reprint of the original 1st ed. 2009
Experimental Protocols
Methods in Molecular Biology Series, Vol. 466

Coordinators: Hewitson Tim D., Becker Gavin J.

Language: English

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Kidney Research
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Kidney cancer, experimental research
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276 p. · 15.5x23.5 cm · Paperback

As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology? series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.

Isolation and Propagation of Cell Populations.- Isolation and Propagation of Glomerular Mesangial Cells.- Isolation and Primary Culture of Human Proximal Tubule Cells.- Propagation and Culture of Renal Fibroblasts.- Animal Models.- Small Animal Models of Kidney Disease: A Review.- Techniques and Applications.- Measurement of Glomerular Filtration Rate in Conscious Mice.- Laser Capture Microdissection of Kidney Tissue.- Quantitative Gene Expression Analysis in Kidney Tissues.- In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy.- Using In Situ Hybridisation to Localize Renal Gene Expression in Tissue Sections.- Immuno and Lectin Histochemistry for Renal Light Microscopy.- Immuno and Lectin Histochemistry for Renal Electron Microscopy.- Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia.- Identification of Apoptosis in Kidney Tissue Sections.- Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction.- Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells.- Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue.- SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue.- In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli.
Includes step-by-step instructions providing a level of detail rarely found in journal articles Provides experimental techniques for examining the mechanisms and pathology of kidney disease Details protocols for isolating and culturing the most common renal cell types Involves an in-depth review of the various animal models of renal disease Emphasizes techniques for mouse models Contains detailed methods for both gene and protein analysis in the kidney Examines methodology for in vivo gene transfer