Description
Legionella (2nd Ed., 2nd ed. 2019)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1921
Coordinators: Buchrieser Carmen, Hilbi Hubert
Language: EnglishSubject for Legionella:
Description
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Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.
Part I: Ecology, Physiology and Genetics of Legionella
1. Analysis of Iron Requirements and Siderophore Production
Denise M. Burnside, and Nicholas P Cianciotto
2. The Pathometabolism of Legionella Studied by Isotopologue Profiling
Klaus Heuner, Mareike Kunze, Fan Chen, and Wolfgang Eisenreich
3. Quantification of Viable but Non-culturable Cells of Legionella pneumophila
Rosalie S. Braun, Nilmini Mendis, Laam Li, and Sebastien P. Faucher
4. Selection of Legionella Virulence-related Traits by Environmental Protozoa
Francisco Amaro and Howard Shuman
5. Migration of Acanthamoeba castellanii through Legionella Biofilms
Ramon Hochstrasser and Hubert Hilbi
6. Scar-free Genome Editing in Legionella pneumophila
Nathalie Bailo, Hussein Kanaan, Elisabeth Kay, Xavier Charpentier, Patricia Doublet, and Christophe Gilbert
7. Querying Legionella Genomes using Transposition-sequencing
Léo Hardyand Xavier Charpentier
8. Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq
Stephanie R. Shames
9. Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae
Elizabeth Korevaar, Chen Ai Khoo, and Hayley J. Newton
Part II: Cellular Microbiology of Legionella
10. Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium
Amanda Welin, Stephen Weber, and Hubert Hilbi
11. Sorting of Phagocytic CellsInfected with Legionella pneumophila
Monica Rolandoand Carmen Buchrieser
12. Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment
Nicolas Personnic, Bianca Striednig, and Hubert Hilbi
13. Mitochondrial Dynamics and Activity in Legionella-Infected Cells
Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, and Pedro Escoll
14. Perturbation of Legionella Cell Infection by RNA Interference
Bernhard Steiner, A. Leoni Swart, and Hubert Hilbi
Part III: Biochemical Assays to Study Legionella Effectors and Enzymes
15. Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila
Tomoko Kubori and Hiroki Nagai
16. In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography
Debnath Ghosal, Mohammed Kaplan, Yi-Wei Chang, and Grant J. Jensen
17. Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins
Jiazhang Qiu and Zhao-Qing Luo
18. Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila
Nadezhda Levanova, Irina Tabakova, Thomas Jank, and Yury Belyi
19. Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification
Ernest C. So, Aurélie Mousnier, Gad Frankel, and Gunnar N. Schroeder
20. Inflammasome Activation in Legionella-infected Macrophages
Danielle P. A. Mascarenhas and Dario S. Zamboni
Part IV. Immunity and Host Response Against Legionella
21. Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophilaLina Scheithauer and Michael Steinert
22. The Galleria mellonella InfectionModelfor Investigating the Molecular Mechanisms of Legionella Virulence
Gad Frankeland Gunnar N. Schroeder
23. Dictyostelium Host Response to Legionella Infection: Strategies and Assays Salvatore Bozzaro, Simona Buracco, Barbara Peracino, and Ludwig Eichinger
24. 6.1.3 The Caenorhabditis elegans model of Legionella infection
Ann Karen C. Brassinga and Costi D. Sifri
25. The Mouse as a Model for Pulmonary Legionella Infection
Garrett Z. Ng, Anastasia Solomatina, Ian R. van Driel, and
Elizabeth L. Hartland
Part V. Genomics, Transcriptomics, and Proteomics of Legionella
26. Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples
Vincent Delafont, Yoann Perrin, Didier Bouchon, Laurent Moulin, and Yann Héchard
27. Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients
Ana Elena Pérez-Cobas and Carmen Buchrieser
28. Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry
Sandra Maaß, Gina Moog, and Dörte Becher
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts