Metabotropic Glutamate Receptor Technologies, 1st ed. 2021 Neuromethods Series, Vol. 164

This volume highlights various techniques used to study multiple aspects of mGlu receptor function. Chapters in this book cover topics such as modern approaches for the development and screening of allosteric modulators; fluctuations in intracellular calcium induced by mGlu receptor activation; dopamine-glutamate interactions in the central nervous system; methods for assessing mGlu receptor localization; and common techniques for evaluating mGlu modulation in behavior in rodents, including stress and alcohol drinking. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and practical, Metabotropic Glutamate Receptor Technologies provides researchers with a starting point to address important questions regarding mGlu and its importance. Researchers working in the fields of neuroscience and physiology of multiple peripheral systems will find this volume most useful. 

Series Preface…

Preface…

Table of Contents…

Contributors …

1. Technologies for Screening of mGlu Receptor Allosteric Modulators

Alice L. Rodriguez and Colleen M. Niswender

2. Positron Emission Tomography (PET) Imaging of Metabotropic Glutamate Receptor Subtype 2 (mGlu2) Based on a Negative Allosteric Modulator Radioligand

Xiaofei Zhang, Zhiwei Xiao, Katsushi Kumata, Tomoteru Yamasaki, Lee Josephson, Ming-Rong Zhang, Lu Wang, and Steven H. Liang

3. PET Imaging of Type 5 Metabotropic Glutamate Receptors

Stephanie G. Scala, Kelly Smart, Sylvia M.L. Cox, Chawki Benkelfat and Marco Leyton

4. Electrophysiological and Calcium Imaging Approaches to Study Metabotropic Glutamate Receptors

Philippe Coulon

5. Role of Metabotropic Glutamate Receptors (mGluRs) in the Regulation of Intracellular Calcium Signaling: Theory, Protocols, and Data Analysis

Vaibhav Dhyani, Sarpras Swain, Rishikesh Kumar Gupta, Abha Saxena, Ranjana Singh, and Lopamudra Giri

6. Optical Approaches for Modulating mGlu Receptor Activity

Lauren E. Hood, Jonna M. Leyrer-Jackson, and M. Foster Olive

7. Classic and Modern Approaches to Investigating Interactions between Dopamine Systems and Metabotropic Glutamate Receptors

Kari A. Johnson

8. Using Western Blotting for the Evaluation of Metabotropic Glutamate Receptors within the Nervous System: Procedures and Technical Considerations

Jonna M. Leyrer-Jackson, Mark D. Namba, Lauren E. Hood, and M. Foster Olive

9. Investigation of mGlu Receptor Cell Surface Trafficking in Acute Brain Slices Using Biotinylation

Marek Schwendt

10. Co-Labeling of Neuronal Cells using DiI Neuronal Filling and Immunohistochemistry to Explore Metabotropic Glutamate Receptor Expression

Jonna M. Leyrer-Jackson, Sade Spencer, and Cassandra D. Gipson

11. Fluorescence Immunohistochemistry Techniques for Labelling Metabotropic Glutamate Receptors in the Central Nervous System

Ryan S. Pevey, Jonna M. Leyrer-Jackson, Erin K. Nagy, and M. Foster Olive

12. Multiplex Arrays for Quantifying Relative Levels of Cytokines in Brain Tissue Lysates: Implications for mGlu Receptor Mediated Alterations

Lauren E. Hood, Jonna M. Leyrer-Jackson, Erin K. Nagy, and M. Foster Olive

13. mGlu Receptor Modulation in Murine Models of Stress and Affective Disorders

James Maksymetz and Max E. Joffe