Description
Patch Clamp Electrophysiology, 1st ed. 2021
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2188
Language: EnglishSubjects for Patch Clamp Electrophysiology:
Publication date: 10-2021
333 p. · 17.8x25.4 cm · Paperback
Publication date: 10-2020
333 p. · 17.8x25.4 cm · Hardback
Description
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This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.
1. An Introduction to Patch Clamp Recording
Charlotte L. Hill and Gary J. Stephens
2.Patch Clamp Technology in the 21st Century
Jan Dolzer
3. Heterologous Expression of Ion Channels in Mammalian Cell Lines
Alistair Mathie, Emma L. Veale, and Robyn G. Holden
4. Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel
Ekaterina Zaitseva, Alison Obergrussberger, Conrad Weichbrodt, Mordjane Boukhet, Frank Bernhard, Christopher Hein, Gerhard Baaken, Niels Fertigand Jan C. Behrends
5. Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology
Kadla R. Rosholm, Kim Boddum, and Anders Lindquist
6. Multi-electrode Arrays
Russell Burley and Jenna R M Harvey
7. Utilising Automated Electrophysiological Platforms in Epilepsy Research
Carol J. Milligan and Svenja Pachernegg
8. Dynamic Clamp on a Windows PC
Lorin S. Milescu
9. Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing Kashif Mahfooz and Tommas J. Ellender
10. Bioluminescence Methodology for Ion Channel Studies
Paul A Wadswort,, Aditya K. Singh, Nghi Nguyen, Clifford Stephan, and Fernanda Laezza
11. Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons
Francesco Tamagnini
12. Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method
Timothy W. Church and Matthew G. Gold
13. In Vivo Patch Clamp Studies
Yi Zhou, He Li and Zhongju Xiao
14. In Vivo Optogenetics with Stimulus Calibration
Luke T. Coddington and Joshua T. Dudman
15. Single or Double Patch Clamp Recordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics
Jean Simonnet, Louis Richevaux, and Desdemona Fricker
16. Optogenetics and Optical Tools in Automated Patch Clamping
Kim Boddu, Peder Skafte-Pedersen, Jean-Francois Rolland, and Sandra Wilson
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
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