Peptide Microarrays (2^nd Ed., Softcover reprint of the original 2nd ed. 2016)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1352

This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays. Advancements made in recent years in peptide library synthesis, immobilization chemistry and array production have created a foundation from which different new applications are derived, extending the ways in which peptide microarray technology is applied every day. Divided into three sections, the book covers cutting-edge methods and technology, chemoselective strategies for peptide immobilization, and peptide microarrays for medical applications. Written for the high successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Fully updated and authoritative, Peptide Microarrays: Methods and Protocols, Second Edition seeks to encourage scientists to apply current peptide array protocols to the study of interesting new biochemical and medical questions and to assist researchers aiming at developing new methods to further develop peptide microarray technology.

Part I: Peptide Arrays: Cutting-Edge Methods and Technology

1. Peptide Arrays on Planar Supports

            Víctor Tapia and Rudolf Volkmer

2. High-Throughput Microarray Incubations Using Multi-Well Chambers

            Johannes Zerweck, Ulf Reimer, Janina Jansong, Nikolaus Pawlowski, Christoph Tersch, Maren Eckey, and Tobias Knaute

3. Analysis of Protein Tyrosine Kinase Specificity Using Positional Scanning Peptide Microarrays

            Yang Deng and Benjamin E. Turk

4. Secondary Structure Determination of Peptides and Proteins After Immobilization

            Stella H. North and Chris R. Taitt

5. Peptides and Anti-Peptide Antibodies for Small and Medium Scale Peptide and Anti-Peptide Affinity Microarrays: Antigenic Peptide Selection, Immobilization, and Processing

            Fan Zhang, Andrea Briones, and Mikhail Soloviev

6. Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes

            Ryan McBride, Steven R. Head, Phillip Ordoukhanian, and Mansun Law

7. The Peptide Microarray-Based Resonance Light Scattering Assay for Sensitively Detecting Intracellular Kinase Activity

            Tao Li, Xia Liu, Dianjun Liu, and Zhenxin Wang

8. Anomalous Reflection of Gold: A Novel Platform for Biochips

            Amir Syahir, Kin-ya Tomizaki, Kotaro Kajikawa, and Hisakazu Mihara

9. High Throughput Peptide Screening on a Bimodal Imprinting Chip Through

            Weizhi Wang, Qiaojun Fang, and Zhiyuan Hu

10. Analyzing Peptide Microarray Data with the R pepStat Package

            Gregory Imholte, Renan Sauteraud, and Raphael Gottardo

Part II: Chemoselective Strategies to Peptide Immobilization

11. Chemoselective Strategies to Peptide and Protein Bioprobes Immobilization on Microarray Surfaces

            Alessandro Gori and Renato Longhi

12. Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry

            Denis Prim and Marc E. Pfeifer

13. Clickable Polymeric Coating for Oriented Peptide Immobilization

            Laura Sola, Alessandro Gori, Marina Cretich, Chiara Finetti, Caterina Zilio, and Marcella Chiari

14. Oriented Peptide Immobilization on Microspheres

            Lisa C. Shriver-Lake, George P. Anderson, and Chris R. Taitt

15. A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis

            Kenji Usui, Kin-ya Tomizaki, and Hisakazu Mihara

Part III: Peptide Microarrays for Medical Applications

16. Peptide Microarrays for

            Carsten Grötzinger

17. Synthetic Peptide-Based ELISA and ELISpot Assay for Identifying Autoantibody Epitopes

            Judit Pozsgay, Eszter Szarka, Krisztina Huber, Fruzsina Babos, Anna Magyar, Ferenc Hudecz, and Gabriella Sarmay

18. IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay

            Javier Martínez-Botas and Belén de la Hoz

19. IgE Epitope Mapping Using Peptide Microarray Immunoassay

            Gustavo Gimenez, Sara Benedé, and Jing Lin

20. Spot Synthesis: An Optimized Microarray to Detect IgE Epitopes

            Salvatore G. De-Simone and Paloma Napoleão-Pêgo

21. Mapping of Epitopes Occurring in Bovine α_s1-Casein Variants by Peptide Microarray Immunoassay

            Maria Lisson and Georg Erhardt

Includes cutting-edge methods and protocols for peptide microarray study

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras