Prions, 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1658

This volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Prions: Methods and Protocols aims to ensure successful results in the further study of this vital field.

PART I: Methods for Biochemical and Biophysical Analysis of Prion Disease

Laura J. Ellett

3. Analysis of Prion Protein Conformation using Circular Dichroism Spectroscopy

Laura J Ellett and Vanessa Johanssen

4. Analysis of Prion Protein Structure using Nuclear Magnetic Resonance Spectroscopy

Ivana Biljan, Gregor Ilc, and Janez Plavec

5. Immunodetection of PrP^Sc using Western Immunoblotting Techniques

Gerald S. Baron and Gregory J. Raymond

Shayne A. Bellinghamand Andrew F. Hill

PART II: Methods for Cellular Analysis Of Prion Disease

7. Cell Biology Approaches to Studying Prion Diseases

Suzette A. Priola

Zaira E. Arellano-Anaya, Alvina Huor, Pascal Leblanc, Olivier Andréoletti,and Didier Vilette

9. Generation of Infectious Prions and Detection with the Prion Infected Cell Assay

Laura J Vella, Bradley Coleman, and Andrew F Hill

10. Analysis of Cellular Prion Protein Endoproteolytic Processing

Victoria Lewis

Cathryn L Haigh

12. Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons

Giuseppe D. Ciccotosto, Metta Jana, and Roberto Cappai

PART III: Methods for Cell Free Propagation of Prions

14. RT-QuIC Assays for Prion Disease Detection and Diagnostics

Christina D. Orrù, Bradley R. Groveman, Andrew G. Hughson, Matteo Manca, Lynne D. Raymond, Gregory J. Raymond, Katrina J. Campbell, Kelsie J. Anson, Allison Kraus, and Byron Caughey

15. A Quick Method to Evaluate the Effect of the Amino Acid Sequence in the Misfolding Proneness of the Prion Protein

Natalia Fernández-Borges, Hasier Eraña, Saioa Rodríguez-Elezgarai, Chafik Harrathi, Vanesa Venegas, and Joaquín Castilla

16. Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases

Julie A. Moreno and Glenn C. Telling

17. In vivo Near Infrared Imaging of Neurodegeneration

Victoria A Lawson, Carolin Tumpach, Cathryn L Haigh, and Simon C Drew

18. Strain Typing of Prion Diseases using in vivo Mouse Models

Aileen Boyle, Kris Hogan, Jean C. Manson, and Abigail B Diack

19. Preparation and Immunostaining of the Myenteric Plexus of Prion Infected Mice

Laura J. Ellett and Victoria A. Lawson

PART V: Methods for the Detection and Treatment of Prion Diseases

20. Cell Culture Methods for Screening of Prion Therapeutics

Hilary E. McMahon

21. Real-Time Quaking Induced Conversion for Diagnosis of Prion Disease

Katsuya Satoh, Ryuichiro Atarashi, and Noriyuki Nishida

22. Methods for Molecular Diagnosis of Human Prion Disease

23. Molecular sub-typing of PrP^res in Human Sporadic CJD Brain Tissue

Klug, G.M., Lewis, V., and Collins, S.J.

Part VI: Methods for Investigation of Prion-like Disease

Leslie I. Grad, Edward Pokrishevsky, and Neil R. Cashman

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras