Description
Sedimentation Velocity Analytical Ultracentrifugation
Discrete Species and Size-Distributions of Macromolecules and Particles
Author: Schuck Peter
Language: EnglishSubjects for Sedimentation Velocity Analytical Ultracentrifugation:
Keywords
Sedimentation Coefficient Distribution; Lamm Equation Solutions; Sedimentation Boundaries; Sedimentation Coefficient; SV Data; Molar Mass; Non-diffusing Particles; Sedimenting Species; Solution Column; Frictional Ratio; Radial Dilution; Buoyant Molar Mass; SV Experiment; Lamm Equation; Macromolecular Sedimentation; Molar Mass Distribution; Non-linear Parameters; Face Tough; Data Set; Smax Smin; Partial Specific Volume; Discrete Species; Boundary Fractions; Meniscus Position; Apparent Molar Mass
Publication date: 12-2019
· 17.8x25.4 cm · Paperback
Publication date: 12-2016
· 17.8x25.4 cm · Hardback
Description
/li>Contents
/li>Biography
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A follow-up to the experimental and instrumental aspects described in Basic Principles of Analytical Ultracentrifugation, the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles describes the theory and practice of data analysis. Mathematical models for the sedimentation process and the evolution of detected signals are developed in a comprehensive framework, jointly with the description of current and historical strategies for how to extract from noisy experimental data the physical parameters of interest, such as size, mass, and shape, composition, and polydispersity of sedimenting particles.
The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. A useful reference for researchers and graduate students of macromolecular disciplines, these methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in the volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems.
Software referenced in the book is available for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx
Basic Analysis Principles
Sedimentation of Discrete Non-Interacting Particles
Properties of Sedimentation Coefficient Distributions
Distributions Of Non-Diffusing Particles
Distributions of Diffusing Particles
Sedimentation Coefficient Distributions from Boundary Derivatives and Extrapolations
Multi-Component Distributions
Practical Analysis of Non-Interacting Systems
Appendix A: Numerical Solutions of the Lamm Equation
Appendix B: Calculating Distributions
Dr. Schuck obtained his Ph.D. from the Goethe-University Frankfurt am Main, Germany, where he worked on interactions of integral proteins of the erythrocyte membrane using analytical ultracentrifugation. He received his post-doctoral research training in physical biochemistry with Dr. Allen Minton at NIDDK, and joined the Bioengineering and Physical Science Program of NCRR as a Research Fellow in 1997. He is currently a Earl Stadtman Tenure-Track Investigator and Chief of the Dynamics of Macromoleular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics at the National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health.
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