Description
The Immune Synapse, Softcover reprint of the original 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1584
Coordinators: Baldari Cosima T., Dustin Michael L.
Language: EnglishSubject for The Immune Synapse:
Publication date: 07-2018
Support: Print on demand
Publication date: 03-2017
Support: Print on demand
Description
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This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse, new high resolution imaging, biophysical and computational methods for the study of the immune synapse, effector immune synapses, B cell, NK and mast cell immune synapses, and immune interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Immune Synapse: Methods and Protocols will be of interest to immunologists and, at a more general level, to cell biologists, biophysicists and computational biologists.
1. The Immune Synapse: Past, Present, and Future
Michael L Dustin and Cosima T Baldari
2. Analyzing Actin Dynamics of the Immunological Synapse
Katarzyna I Jankowska and Janis K Burkhardt
3. Analysis of Microtubules and Microtubule-Organizing Centre at the Immune Synapse
Noelia Blas-Rus, Eugenio Bustos-Morán, Francisco Sánchez-Madrid, and Noa B Martín-Cófreces
4. Analyzing the Dynamics of Signaling Microclusters
Akiko Hashimoto-Tane, Tadashi Yokosuka, and Takashi Saito
5. Reconstitution of TCR Signaling using Supported Lipid Bilayers
Xiaolei Su, Jonathon A Ditlev, Michael K Rosen, and Ronald D Vale
6. Plasma Membrane Sheets for Studies of B Cell Antigen Internalization From Immune Synapses
Carla R. Nowosad and Pavel Tolar
7. Studying The Dynamics Of TCR Internalization At The Immune Synapse
Enrique Calleja, Balbino Alarcón, and Clara L Oeste
8. T Cell Receptor Activation of NF-Κb in Effector T Cells: Visualizing Signaling Events Within and Beyond the Cytoplasmic Domain of the Immunological Synapse
Maria Traver, Suman Paul, and Brian Schaefer
9. Imaging Vesicular Traffic at the Immune Synapse
Jérôme Bouchet, Iratxe Del Río-Iñiguez, and Andrés Alcover
10. Analysis of TCR Recycling at the Immune Synapse
11. Simultaneous Membrane Capacitance Measurements and TIRF Microscopy to Study Granule Trafficking at Immune Synapses
Marwa Sleiman, David R. Stevens, and Jens Rettig
12. Mathematical Modeling of Synaptic Patterns
Anastasios Siokis, Philippe Robert, and Michael Meyer-Hermann
13. Super-Resolution Analysis of TCR-Dependent Signaling: Single Molecule Localization Microscopy
Valerie A Barr, Jason Yi, and Lawrence E Samelson
14. Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse
Gerhard Schütza and Johannes B Huppa
15. Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes
Lining Ju, Yunfeng Chen, Muaz Nik Rushdi, Wei Chen, and Cheng Zhu
16. Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains during Immunological Synapse Formation using Donor-Quenching FRET and FLIM-FRET
17. Revealing the Role of Microscale Architecture in Immune Synapse Function through Surface Micropatterning
Joung-Hyun Lee and Lance C Kam
18. Spatial Control of Biological Ligands on Surfaces
Haogang Cai, David Depoil, James Muller, Michael P.Sheetz, Michael L Dustin, and
Shalom J Wind
19. Probing Synaptic Biomechanics using Micropillar Arrays
Weiyang Jin, Charles T Black, Lance C Kam, and Morgan Huse
20. Microchannels for Study of Immunological Synapses and Kinapses
Hélène D Moreau, Philippe Bousso, and Ana-Maria Lennon-Duménil
21. Purification of LAT Containing Membranes from Resting and Activated T Lymphocytes
Claire Hivroz,Paola Larghi, Mabel Jouve, and Laurence Ardouin
22. Quantitative Phosphoproteomic Analysis of T-Cell Receptor Signaling
Nagib Ahsan and Arthur R Salomon
23. Imaging Asymmetric T Cell Division
Mirren Charnley and Sarah M Russell
24. Ultrastructure Of Immune Synapses
Jaime Llodrá
25. Systems Imaging of the Immune Synapse
Rachel Ambler, Xiangtao Ruan, Robert F Murphy, and Christoph Wülfing
26. Comprehensive Analysis of Immunological Synapse Phenotypes using Supported Lipid Bilayers
Salvatore Valvo, Viveka Mayya, Jehan Afrose, Elena Seraia, Daniel Ebner, and Michael L. Dustin
27. Studying Immunoreceptor Signaling in Human T Cells using Electroporation of In Vitro Transcribed mRNA
Omkar Kawalekar, Carl H June, and Michael C. Milone
28. A Protein Expression Tool-Kit for Studying Signalling in T Cells
Ana Mafalda Santos, Jiandong Huo, Deborah Hatherley, Mami Chirifu, and Simon J Davis
29. Imaging The Effector CD8 Synapse
Gordon L Frazer, Yukako Asano, and Gillian M Griffiths
30.The Mast Cell Antibody-Dependent Degranulatory Synapse
Salvatore Valitutti, Régis Joulia, and Eric Espinosa
31. Measurement of Lytic Granule Convergence after Formation of an NK Cell Immunological Synapse
Hsiang-Ting Hsu, Alexandre F Carisey, and Jordan S Orange
32. Studying the T Cell-Astrocyte Immune Synapse
George P Cribaro, Elena Saavedra-Lopez, Paola V Casanova, Laura Rodríguez, and Carlos Barcia
33. Aberrant Immunological Synapses Driven by Leukemic Antigen Presenting Cells
Fabienne Mcclanahan and John G Gribben
34. Studying the Immune Synapse in HIV-1 InfectionIratxe Del Río-Iñiguez, Jérôme Bouchet, and Andrés Alcover
35. In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes
Hélène D. Moreau and Philippe Bousso
36. Studying Dendritic Cell-T Cell Interactions Under In Vivo Conditions
Nicholas Van Panhuys
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts