Vaccines for Invasive Fungal Infections, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1625

Part I: Discovery and Testing of Vaccine Candidates

1. Mouse Immunization with Radioattenuated Yeast Cells of Paracoccidioides brasiliensis

            Estefânia Mara do Nascimento Martins and Antero Silva Ribeiro de Andrade

2. Heat-Killed Yeast as a Pan-Fungal Vaccine

            Marife Martinez, Karl V. Clemons, and David A. Stevens

3. Immunoinformatics as a Tool for New Antifungal Vaccines

            Rupanjali Chaudhuri and Srinivasan Ramachandran

4. Rational Design of T Lymphocyte Epitope-Based Vaccines Against Coccidioides Infection

            Brady J. Hurtgen and Chiung-Yu Hung

5. Identification of Fungal T Cell Epitopes by Mass Spectrometry-Based Proteomics

            Bernd Roschitzki and Salomé LeibundGut-Landmann

6. Intranasal Antifungal Vaccination Using DNA-Transfected Dendritic Cells

            Shanjana Awasthi

7. DNAhsp65 Vaccine as Therapy Against Paracoccidioidomycosis

            Alice M. Ribeiro, André C. Amaral, Maria Sueli S. Felipe, and Anamelia L. Bocca

8. Idiotypic Antifungal Vaccination: Immunoprotection by Antiidiotypic Antibiotic Antibodies

            Luciano Polonelli, Walter Magliani, and Stefania Conti

9. Peptide Vaccine Against Paracoccidioidomycosis

            Carlos P. Taborda and Luiz R. Travassos

10. Methodology for Anti-Cryptococcal Vaccine Development

            Ashok K. Chaturvedi and Floyd L. Wormley Jr.

Part II: Design and Delivery

11. Beta-Glucan Particles as Vaccine Adjuvant Carriers

12. Th1-Inducing Agents in Prophylaxis and Therapy for Paracoccidioidomycosis

            Thiago Aparecido da Silva, Fabrício Freitas Fernandes, Taise Natali Landgraf, Ademílson Panunto-Castelo, and Maria Cristina Roque-Barreira

13. Nanoparticle-Based Mycosis Vaccine

            Swaleha Zubair, Asim Azhar, Nazoora Khan, Ejaj Ahmad, Mohd Ajmal, and Owais Mohammad

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14. Yeast Expressing Gp43 Protein as a Vaccine Against Paracoccidioides brasiliensis Infection

            Aline Ferreira Oliveira and Paulo S.R. Coelho

15. Vaccination with Phage-Displayed Antigenic Epitope

            Yicun Wang and Li Wang

16. Preparation of an Oral Vaccine by Proteome Analysis and Molecular Display Technology

            Seiji Shibasaki and Mitsuyoshi Ueda

Part III: Endpoint Assessment

17. Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System

            Chi Zhang and Ling Lu

18. Endpoint Assessment in Rabbit Models of Invasive Pulmonary Aspergillosis and Mucormycosis

            Vidmantas Petraitis, Ruta Petraitiene, William Hope, and Thomas J. Walsh

19. CD4⁺ T Cells Mediate Aspergillosis Vaccine Protection

            Diana Diaz-Arevalo and Markus Kalkum

20. T-Cell-Mediated Cross-Protective Immunity

            Nina Khanna and Claudia Stuehler

21. Assessment of Post-Vaccination Phagocytic Activation Using Candida albicans Killing Assays

22. Immunization with Antigen-Pulsed Dendritic Cells Against Highly Virulent Cryptococcus gattii Infection: Analysis of Cytokine-Producing T Cells

            Keigo Ueno, Makoto Urai, Shogo Takatsuka, Masahiro Abe, Yoshitsugu Miyazaki, and Yuki Kinjo

Part V: Translation to Clinical Use

23. Testing Antifungal Vaccines in an Animal Model of Invasive Candidiasis and in Human Mucosal Candidiasis

Includes cutting-edge techniques for the study of invasive mycosis vaccine creation and implementation

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts