Bioenergetics, 1990
Molecular Biology, Biochemistry, and Pathology

The emergence of the Biochemical Sciences is underlined by the FAOB symposium in Seoul and highlighted by this Satellite meeting on the "New Bioenergetics. " Classical mitochondrial electron transfer and energy coupling is now complemented by the emerging molecular biology of the respiratory chain which is studied hand in hand with the recognition of mitochondrial disease as a major and emerging study in the basic and clinical medical sciences. Thus, this symposium has achieved an important balance of the fundamental and applied aspects of bioenergetics in the modern setting of molecular biology and mitochondrial disease. At the same time, the symposium takes note not only of the emerging excellence of Biochemical Studies in the Orient and indeed in Korea itself, but also retrospectively enjoys the history of electron transport and energy conservation as represented by the triumvirate ofYagi, King and Slater. Many thanks are due Drs. Kim and Ozawa for their elegant organization of this meeting and its juxtaposition to the FAOB Congress. Britton Chance April 2, 1990 v PREFACE This book contains the contributed papers presented at the "International Symposium on Bioenergetics: Molecular Biology, Biochemistry and Pathology", held in Seoul, Korea, August 18-21, 1989, sponsored by International Union of Biochemistry (as ruB Symposium No. 191) and Ewha Womans University, Seoul, Korea. The symposium was held in honor of Professor Kunio Yagi to commemorate his 70th birthday.

Opening Session.- Introductory Remarks.- Opening Lecture.- Special Session.- Memories of My Flavoprotein Research.- I. Biochemistry 1. Mitochondrial Electron Transfer.- An Overview of Ubiquinone Proteins.- Studies on the Mechanism of Action of the Mitochondrial Energy-Linked Nicotinamide Nucleotide Transhydrogenase.- Structures of Beef Mitochondrial Complex I Subunits.- Inhibition of the Mitochondrial Complex I Activity by Fatty Acid Derivatives of Vanillylamide.- New 4-Hydroxypyridine and 4-Hydroxyquinoline Derivatives as Inhibitors of NADH-Ubiquinone Oxidoreductase in the Respiratory Chain II.- Effects of Redox State for Ubiquinone-10 on the Membrane Fluidity of Cardiolipin-Containing Liposomes.- Features of Assembly and Mechanism of Yeast Mitochondrial Ubiquinol: Cytochrome c Oxidoreductase.- Structural Studies of Euglena Cytochrome c1.- How Well is Cytochrome c Engineered?.- Subunit I is the Catalytic Center of P. denitrificans Cytochrome c Oxidase.- The Separation between Cytochrome a and Cytochrome a3 in the Absolute Spectrum.- I. 2. Energy Coupling and Ion Transport.- Which Electron-Transferring Reactions in the Respiratory Chain Contribute to the Energy Conservation?.- Cytochrome Oxidase and Peroxide Metabolism.- Do Hydrogen Ions Serve as Bioenergetic Messengers in Yeast?.- Molecular Organization and Regulation of the Protonmotive System of Mammalian ATP Synthase.- Situation of Archaebacterial ATPase among Ion-Translocating ATPases.- Structure and Chemical Modification of Pig Gastric (H++K+)-ATPase.- Inhibition of the Uncoupler-Induced Mitochondrial ATP-Hydrolysis by the Cooperative Work of the ATPase Inhibitor, 9K Protein and 15K Protein.- Reconstitution of H+ATPase into Planar Phospholipid Bilayers and its Kinetic Analysis.- A Mitochondrial Carrier Family for Solute Transport.- The Calcium Pump of the Plasma Membrane: Structure/Function Relationships.- I.3. Other Related Topics.- The 9-kDa Polypeptide with Iron-Sulfur Centers A/B in Spinach Photosystem I with Special Reference to its Structure and Topographic Consideration in Thylakoid Membrane.- A Photo-Signal Transducing Photoreceptor (Stentorin) in Stentor coeruleus: A Brief Review.- Theory and Applications of the Kinetics of Substrate Reaction during Irreversible Modification of Enzyme Activity.- High Pressure Kinetic Studies on Certain Hemoproteins.- The Purification and Properties of Glycerol-3-Phosphate Dehydrogenase in the Mitochondrial Inner Membrane.- II. Molecular Biology.- Subunit 8 of Yeast Mitochondrial ATP Synthase: Biochemical Genetics and Membrane Assembly.- Gene Structure of Human ATP Synthase Beta Subunit.- Molecule and Gene of Sulfolobus acidocaldarius ATPase.- Nuclear Genes Encoding Two Subunits of Human Mitochondrial Cytochrome bc1 Complex, Cytochrome c1 and Ubiquinone-Binding Protein: Their Structural Organization of the 5?-Flanking Regions and Chromosomal Localization.- Studies on the Function of the Mitochondrial Hinge Protein: Molecular Genetic Approach Using Yeast as a Model System.- Molecular Evolution and Biology of Human Mitochondrial DNA.- III. Mitochondrial Pathology.- Stable and Unstable Bioenergetics in Vivo.- Regulation of Activity and Tissue-Specific Expression of Cytochrome c Oxidase Genes under Normal and Pathological Conditions.- Mitochondrial DNA Mutations as an Etiology of Human Degenerative Diseases.- Mitochondrial Myopathies: Morphological Approach to Molecular Abnormalities.- S1 Nuclease Analysis and Direct Sequencing of Deleted Mitochondrial DNA in Myopathic Patients: Role of Directly Repeated Sequences in Deletion.- Thirty Years of Mitochondrial Pathophysiology: From Luft's Disease to Oxygen Toxicity.- Participants.- Author Index.