Biomedical Electron Microscopy
Illustrated Methods and Interpretations

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Language: English

131.23 €

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548 p. · 21.4x27.7 cm · Hardback
This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.

Micrograph InterpretationFixativesFixative VehicleFixative ApplicationDehydration and EmbeddingFreezing and Freeze-SubstitutionSupport FilmsUltramicrotomySection-StainingMicroscopyImage RecordingPhotographic and Digital PrintingNegative StainingAutoradiographyCytochemistryImmunocytochemistryFreeze-Fracturing and ShadowingSampling and QuantitationImage Processing3D ReconstructionsAppendix: Practical Methods

AUDIENCE: Undergraduates, graduate students, and researchers in cell biology, developmental biology, microbiology, pathology, cancer research, structural biology, and endocrinology, research technicians.
  • Authored by the key leaders in the biological electron microscopy field
  • Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines
  • Introduces students on how to read and interpret electron micrographs