Description
Caenorhabditis Elegans (2nd Ed.)
Molecular Genetics and Development
Methods in Cell Biology Series
Directors of collection: Rothman Joel, Singson Andrew
Language: EnglishSubject for Caenorhabditis Elegans:
504 p. · 19x23.3 cm · Hardback
Description
/li>Contents
/li>Readership
/li>Comment
/li>
An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.
Culturing methods and genetic proceduresGeneral methods of culture: Jim LewisMutagenesis: Phil AndersonGenetic screening methods: James Thomas, Eric Jorgensen, Eric LambieGenetic mapping methods: Ben Williams, Stephen Wicks (Boston College)Genetic mosaic analysis: Robert HermanUse of specialized chromosomes and rearrangements: Ann Rose, David BaillieGene knockouts: Shohei Mitani, Robert BarsteadRNAi and RNAi screening methods: Julie Ahringer, Andrew Dillin
Genomics and BioinformaticsTranscriptional profiling: Stuart Kim, Don Moerman, Valerie ReinkeMicroRNA methods: Victor Ambros, Frank Slack, Amy PasquinelliAnalysis of RNA function: Marv Wickens, Judith KimbleAnalysis of the interactome: Marc VidalWormBase: Eric Schwartz, Paul Sternberg, Lincoln SteinStructural genomics: Ming Luo, Larry DeLucas, Songlin Li, Mike Carson (U. Alabama)Proteomics: Ahna Skop, Moerman D., Isobe T, Krijgsveld, JTranscriptional regulatory networks: Marion Walhout
Markers for gene expression and protein localizationImmunofluorescence and antibodies: David MillerImaging: John White, William MohlerElectron Microscopy: David HallProteins and protein assemblies: Henry EpsteinAnalysis of morphogenesis: Jeff Hardin, Andrew ChisholmCell death: Monica Driscoll, Ding Xue
Researchers and students in cell, molecular and developmental biology
- Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
- Chapters written by leaders in the field
- Tried and true methods deliver busy researchers a one-stop compendium of essential protocols