Cell-free DNA as Diagnostic Markers, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1909

This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Part I: An Overview

1. Cell Free DNA: Applications in Different Diseases

Rossella Ranucci

2. Cell Free DNA: An Overview of Sample Types and Isolation Procedures

Part II: A Liquid Biopsy for Cancer Disease

3. Circulating Cell Free DNA and Cancer Therapy Monitoring: Methods and Potential

4. Cell Free DNA in the Liquid Biopsy Context: Role and Differences between ctDNA and CTC Marker in Cancer Management

Pietro Fici

Part III: Methods in Cancer

Sara Ravaioli

6. Copy Number Variation Analysis on Cell Free Serum DNA

Heidi Schwarzenbach

7. Studying Copy Number Variations in Cell Free DNA: The Example of AR in Prostate Cancer

Samanta Salviand Valentina Casadio

Takashi Takeshita and Hirotaka Iwase

9. From cfDNA to Sequencing: Workflows and Potentials

Michela Tebaldiand Samanta Salvi

Part IV: Epigenetic Modifications

10. Epigenetic Characterization of Cell Free DNA

11. Quantitative Methylation-specific PCR: A Simple Method for Studying Epigenetic Modifications of Cell Free DNA

Luca Sigalotti, Alessia Covre, Francesca Colizzi, and Elisabetta Fratta

Part V: Fetal and Pediatric Diseases

12. Cell Free DNA in Pediatric Rhabdomyosarcoma: Potential and Challenges

Lucia Tombolan, Angelica Zin, and Gianni Bisogno

13. Bioinformatics Pipeline for Accurate Quantification of Fetal DNA Fraction in Maternal Plasma

Meng Ni, Xianlu Laura Peng, and Peiyong Jiang

Part VI: Physical Activity

14. Circulating Cell-Free DNA in Physical Activities

LN Vittori, A. Tarozzi, and P. Maietta Latessa

15. Urinary Cell Free DNA: Potential and Applications

16. Urinary Cell Free DNA: Isolation, Quantification, Quality Assessment

Valentina Casadioand Samanta Salvi

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts