Cell Neurobiology Techniques, Softcover reprint of the original 1st ed. 1999 Neuromethods Series, Vol. 33

The cutting-edge techniques detailed here include those that are particularly popular in multidisciplinary neuroscience research. There are readily reproducible methods for establishing neural cell cultures, measuring enzymes and their inhibitors, and using quantitative autoradiography to study monoamine uptake sites and receptors in the brain. Additional methods cover the use of flow cytometry to study developmental neurobiology, applications of magnetic resonance spectroscopy (MRS) to human brain metabolism, and the study of drug metabolism. Together with its companion volumes, In Vivo Neuromethods and In Vitro Neurochemical Techniques, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.

Quantitative Autoradiography of Monoamine Uptake Sites and Receptors in Rat and Mouse Brain, Tomás A. Reader and Catherine Strazielle. Neural Cell Culture Techniques, Bernhard H. J. Juurlink and Wolfgang Walz. Neural Cell Adhesion Molecules, Kim Tieu and Peter H. Yu. On the Measurement of Enzymes and their Inhibitors, Andrew Holt. Cytochrome P-450 Enzymes: In Vitro Assessment and Clinical Implications, Paul Glue and Robert P. Clement. c-fos Expression as a Marker of Functional Activity in the Brain: Immunohistochemistry, Teresa L. Krukoff. Immediate-Early Genes as Activity Markers in the CNS, George S. Robertson. Principles of Drug Metabolism, with an Emphasis on Psychiatric Drugs, Ronald T. Coutts, Jian Fang, Michael Bourin, and Glen B. Baker. Flow Cytometric Strategies to Study CNS Development, Dragan Maric, Irina Maric, and Jeffrey L. Barker. Neurochemistry of Human Postmortem Brain, Gavin P. Reynolds and Clare L. Beasley. Applications of Proton MRS to Study Human Brain Metabolism, Christopher C. Hanstock and Peter S. Allen. Index.