Cell Reprogramming, Softcover reprint of the original 1st ed. 2015
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1330

This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates; alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians and animal care technicians involved with reprogramming, nuclear transfer and transgenesis.

Part I Background

1. Cellular Reprogramming in Basic and Applied Biomedicine: The Dawn of Regenerative Medicine
Wendy Dean

Part II De Novo Reprogramming

2. Synthetic mRNA Reprogramming of Human Fibroblast Cells
Jun Liu and Paul J. Verma

3. MicroRNA Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells
Shelley E. Swing and Bhanu Prakash V.L. Telugu

4. Generation of Footprint-Free Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmid Vectors
Dmitry A. Ovchinnikov, Jane Sun, and Ernst J. Wolvetang

5. Reprogramming of Human Fibroblasts with Non-Integrating RNA Virus on Feeder-Free or Xeno-Free Conditions
Pauline T. Lieu

Part III Livestock, Domestic and Endangered Species

6. Inducing Pluripotency in Cattle
Luis F. Malaver-Ortega,Amir Taheri-Ghahfarokhi, and Huseyin Sumer

7. Generation of Induced Pluripotent Stem Cells (iPSCs) From Adult Canine Fibroblasts Sehwon Koh and Jorge A. Piedrahita

8. Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a PiggyBac Transposon Delivery System and Temporal Control of Transgene Expression
Kristina Nagy and Andras Nagy

9. Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species
Inbar Friedrich Ben-Nun, Susanne C. Montague, Marlys L. Houck, Oliver Ryder, and Jeanne F. Loring

Part IV Germ-Line Transmission of Pluripotent Stem Cells

10. Generation of Efficient Germ-Line Chimeras Using Embryonic Stem Cells
William A. Ritchie

11. Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation
Lan Kang and Shaorong Gao

12. Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer
Martha C. Gómez and C. Earle Pope

13. Generation of Chimeras from Porcine Induced Pluripotent Stem Cells
Franklin D. West, Steve L. Terlouw, John R. Dobrinsky, Yangqing Lu, Erin T. Jordan,and Steven L. Stice

14. A Novel Method on Somatic Cell Nuclear Transfer with the Minimum Equipment
Hosseini SM, Moulavi F, and Nasr-Esfahani MH

15. Neonatal Care and Management of Foals Derived by Somatic Cell Nuclear Transfer Aime K. Johnson and Katrin Hinrichs

Part V Influencing Reprogramming and Genome Editing

16. Isolation of Reprogramming Intermediates During Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts
Christian M. Nefzger, Sara Alaei, and Jose M. Polo

17. Analysis of Mitochondrial DNA in Induced Pluripotent and Embryonic Stem Cells
William Lee, Richard D. W. Kelly, Ka Yu Yeung, Gael Cagnone, Matthew McKenzie, Justin C. St. John

18. Genome Modification of Pluripotent Cells By Using Transcription Activator-Like Effector Nucleases (TALENs)
Amir Taheri-Ghahfarokhi, Luis F. Malaver-Ortega, and Huseyin Sumer

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts