Cyclin-Dependent Kinase (CDK) Inhibitors, Softcover reprint of the original 1st ed. 2015
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1336

This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.

1. Immunoprecipitation of CDK-Cyclin Complexes for Determination of Kinase Activity
Edurne Gallastegui and Oriol Bachs

2. Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation
Edurne Gallastegui and Oriol Bachs

3. Expression and Purification of Recombinant CDKs: CDK7, CDK8 and CDK9
Reena Pinhero and Krassimir Yankulov

4. Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination
Asterios I. Grigoroudis and George Kontopidis

5. Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors
Sunil Kumar Tripathi, Poonam Singh, and Sanjeev Kumar Singh

6. Protein-Protein Interaction Inspire the De Novo Design of Cyclin-Dependent Kinases
Peptide Inhibitors
Arumugasamy Karthiga, Sunil Kumar Tripathi, Poonam Singh, and Sanjeev Kumar Singh

7. Identification of Cyclin A Binders with a Fluorescent Peptide Sensor
Elena Pazos, José L. Mascareñas and M. Eugenio Vázquez

8. Cell Synchronization Techniques to Study the Action of CDK Inhibitors
Beatriz Pérez-Benavente and Rosa Farràs

9. Analysis of CDK Inhibitors Action on Mitochondria-Mediated Apoptosis
Anna Gortat

10. Evaluating the Effects of CDK Inhibitors in Ischemia-Reperfusion Injury Models
Tatiana Guevara

11. Assessing Cell Cycle Independent Function of the CDK Inhibitor p21^CDKN1A in DNA Repair
Ilaria Dutto, Micol Tillhon, and Ennio Prosperi

12. Drug Delivery Strategies of Chemical CDK Inhibitors
Daniel Alvira and Laura Mondragón

13. Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs
Sanjiv Risal, Deepak Adhikari, and Kui Liu

14. Evaluating Chemical CDK Inhibitors as Cell Death Inducers
Hiroshi Hirai and Yoko Nakatsuru

15. Models for the Study of the Crosstalk between Inflammation and Cell Cycle
Laura J. Hoodless, Calum T. Robb, Jennifer M. Felton, Carl S. Tucker, and Adriano G. Rossi

16. Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors
Martina Palomino-Schätzlein and Antonio Pineda-Lucena

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts