Cytoskeleton Proteins, 1995
A Purification Manual
Coll. Springer Lab Manuals

The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.

A General.- I User’s Instructions.- II General Lab Equipment.- III Preparation of Buffers.- IV Ammonium Sulfate Precipitation.- V Chromatographic Fractionation.- VI Centrifugation.- VII Abbreviations.- B Purification Protocols.- I Actin and Actin-Associated Proteins.- II Tubulin and Microtubule-Associated Proteins.- III Motor Proteins.- IV Intermediate Filament Proteins.- V Plasmamembrane and Organelle-Associated Proteins.- C Suppliers List.

General. User's instructions. General lab equipment. Preparation of buffers. Ammonium sulfate precipitation. Chromatographic fractionation. Centrifugation. Abbreviations. Purification protocols. Actin and actin-associated proteins. Tubulin and microtubule-associated proteins. Motor proteins. Intermediate filament proteins. Plasmamembrane and organelle-associated proteins.