Dopamine Receptor Technologies, Softcover reprint of the original 1st ed. 2015
Neuromethods Series, Vol. 96

To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-transcriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful.

Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery.

Part I: Genetic, Epigenetic, and Post-Transcriptional Analysis of Dopamine Receptors

1. Genetic and Epigenetic Methods for Analysis of the Dopamine D2 Receptor Gene

            Laura M. Fiori, Mireille Daigle, and Paul R. Albert

2. Characterization of D1 Dopamine Receptor Post-Transcriptional Regulation

            Eldo V. Kuzhikandathil

Part II: Computational and Biochemical Methods in the Investigation of Dopamine Receptor Structure, Binding, and Post-Translational Regulation

3. Computational Approaches in the Structure-Function Studies of Dopamine Receptors

            Mayako Michino and Lei Shi

4. Cell-Free Protein Synthesis and Purification of the Dopamine D2 Receptor

            Dipannita Basu, Ritesh Daya, Christal D.R. Sookram, and Ram K. Mishra

5. Wnt Ligand Binding to and Regulation of Dopamine D2 Receptors

            Sehyoun Yoon, Mi-Hyun Choi, and Ja-Hyun Baik

6. Regulation of Pre- and Postsynaptic Protein Phosphorylation by Dopamine D2 Receptors

            Alessandra Bonito-Oliva, Giada Spigolon, and Gilberto Fisone

7. Study of Dopamine D1 Receptor Regulation by G Protein-Coupled Receptor Kinases Using Whole-Cell Phosphorylation and Cross-Linking Methods

            Keyvan Sedaghat, Boyang Zhang, Xiaodi Yang, Caroline Lefebvre, and Mario Tiberi

8. Ubiquitination of Dopamine Receptor Studied by Sequential Double Immunoprecipitation

            Kamila Skieterska, Pieter Rondou, and Kathleen Van Craenenbroeck

Part III: Visualization and Imaging of Dopamine Receptors and Ligands

9. Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors

            Lionel Froux, Diana Suarez-Boomgaard, Jerome Baufreton, Alicia Rivera, Maurice Garret, and Anne Taupignon

10. MALDI Mass Spectrometry Imaging of Dopamine and PET D1 and D2 Receptor Ligands in Rodent Brain Tissues

            Richard J.A. Goodwin, Mohammadreza Shariatgorji, and Per E. Andren

11. Positron Emission Tomography Imaging of Dopaminergic Receptors in Rats

            Boguslaw Szczupak and Abraham Martín

Part IV: Molecular and Cell Biological Approaches in the Study of Dopamine Receptor Function

12. Transactivation of Receptor Tyrosine Kinases by Dopamine Receptors

            Jeff S. Kruk, Azita Kouchmeshky, Nicholas Grimberg, Marina Rezkella, and Michael A. Beazely

13. Dopamine Receptors in Human Embryonic Stem Cell Differentiation

            Glenn S. Belinsky, Mandakini B. Singh, Katerina D. Oikonomou, Michele L. McGovern, and Srdjan D. Antic

14. Calcium and PLC Signaling Through Dopamine Receptors

            Lani S. Chun, R. Benjamin Free, and David R. Sibley

15. Intracellular Trafficking Assays for Dopamine D2-Like Receptors

            Chengchun Min, Mei Zheng, and Kyeong-Man Kim

16. Study of Crosstalk Between Dopamine Receptors and Ion Channels

            Ping Su, Albert H.C. Wong, and Fang Liu

Part V: Behavioral Analysis of Dopamine Function

17. Study of Dopamine Receptor and Dopamine Transporter Networks in Mice

            Victor Gorgievski, Eleni T. Tzavara, and Bruno Giros

18. Optogenetic Regulation of Dopamine Receptor-Expressing Neurons

            T. Chase Francis and Mary Kay Lobo

19. Characterization of D3 Dopamine Receptor Agonist-Dependent Tolerance Property

            Samantha R. Cote and Eldo V. Kuzhikandathil

20. Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography

            Dariusz Žurawek, Agata Faron-Górecka, Maciej Kuśmider, Joanna Solich, Magdalena Kolasa, Kinga Szafran, Katarzyna Kmiotek, Piotr Gruca, Mariusz Papp, and Marta Dziedzicka-Wasylewska

Includes cutting-edge methods and protocols involving dopamine receptor research

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras