Glycosaminoglycans, Softcover reprint of the original 1st ed. 2015
Chemistry and Biology
Methods in Molecular Biology Series, Vol. 1229

Glycosaminoglycans: Chemistry and Biology emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches. Chapters focus on chemical and enzymatic synthesis of GAGs for therapeutic purposes; biophysical and biochemical methods for studying GAG-protein interactions; molecular approaches for modulating and defining GAG biosynthesis; informatics approaches for deciphering GAG code; computational approaches for establishing specific and/or non-specific interactions and genetic and biochemical tools for manipulating/visualizing glycosaminoglycan expression and studying their functions in a variety of model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Glycosaminoglycans: Chemistry and Biology serves as a valuable manual for cutting edge methodologies and practical tips to overcome any obstacles with experimentation pertaining to chemistry and biology of glycosaminoglycans.

Part I: Structure

 1.       Automated Synthesis Of Chondroitin Sulfate Oligosaccharides

Chien-Fu Liang, Heung Sik Hahm, Peter H. Seeberger

2.       Enzymatic Synthesis Of Heparan Sulfate And Heparin

 April Joice, Karthik Raman, Caitlin Mencio, Maritza Quintero, Spencer Brown, Thao Kim Nu Nguyen And Balagurunathan Kuberan

3.       Production Of Size-Defined Heparosan, Heparan Sulfate, and Heparin Oligosaccharides By Enzymatic Depolymerization

Spencer Brown And Balagurunathan Kuberan

4.       Chemical Modification Of Heparin And Heparosan

Karthik Raman, Balagurunathan Kuberan, and Sailaja Arungundram

Caitlin Mencio, Vimal Swarup, Marcus Soliai, and Balagurunathan Kuberan

6.       Preparation Of Isotope Enriched Heparan Sulfate Precursors For Structural Biology Studies Xylophone V. Victor, Vy My Tran, Balagurunathan Kuberan, and Thao Kim Nu Nguyen

7.       Synthesis Of Glycosaminoglycan Mimetics Through Sulfation Of Polyphenols

Rami A. Al-Horani, Rajesh Karuturi, Stephen Verespy III, and Umesh R. Desai

8.       Synthesis Of Selective Inhibitors Of Heparan Sulfate And Chondroitin Sulfate Proteoglycan Biosynthesis

 Caitlin Mencio, Dinesh R. Garud, Balagurunathan Kuberan, nd Mamoru Koketsu

9.       Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical and Spectroscopic Analysis

 Mauro S. G. Pavão

10.   Human Blood Glycosaminoglycans: Isolation and Analysis

Md.Ferdous Anower-E-Khuda, and Koji Kimata

11.   Chromatographic Molecular Weight Measurements For Heparin, Its Fragments And Fractions, and Other Glycosaminoglycans

 Barbara Mulloy and John Hogwood

12.   Mass Spectrometric Methods For The Analysis Of Heparin And Heparan Sulfate

Miroslaw Lech, Ishan Capila, and Ganesh V. Kaundinya

13.   Validated Capillary Electrophoretic Assays For Disaccharide Composition Analysis Of Galactosaminoglycans In Biologic Samples and Drugs/Neutraceuticals

14.   Fast Screening Of Glycosaminoglycan Disaccharides By Fluorophore Assisted Carbohydrate Electrophoresis (Face): Applications To Biologic Samples And Pharmaceutical Formulations

Evgenia Karousou, Athanasia Asimakopoulou, Vassiliki Zafeiropoulou, Manuela Viola, Luca Monti, Antonio Rossi, Alberto Passi, and Nikos Karamanos

15.   Capillary Electrophoretic Analysis Of Isolated Sulfated Polysaccharides To Characterize Pharmaceutical Products

 Zachary Shriver And Ram Sasisekharan

16.   Methods For Measuring Exchangeable Protons In Glycosaminoglycans

Consuelo N. Beecher And Cynthia K. Larive

17.   Heparan Sulfate Structure: Methods To Study N-Sulfation and NDST Action

18.   Analysis Of Hyaluronan Synthase Activity

Davide Vigetti, Evgenia Karousou, Manuela Viola, and Alberto Passi

19.   A Rapid, Non-Radioactive Assay For Measuring Heparan Sulfate C-5 Epimerase Activity Using Hydrogen/Deuterium Exchange-Mass Spectrometry

Ponnusamy Babu, Xylophone V. Victor, Karthik Raman, and Balagurunathan Kuberan

20.   Aggrecan - Approaches To Study Biophysical And Biomechanical Properties

 Hadi Tavakoli Nia, Christine Ortiz, and Alan Grodzinsky

21.   Use Of Flow Cytometry For Characterisation And Fractionation Of Cell Populations Based On Their Expression Of Heparan Sulfate Epitopes

22.   A Transgenic Approach To Live Imaging Of Heparan Sulfate Modification Patterns

Matthew Attreed and Hannes E. BüLow

Part II: Functions

 23.   Informatics Tools To Advance The Biology Of Glycosaminoglycans and Proteoglycans

Lewis J. Frey

24.   Designing ‘High-Affinity, High-Specificity’ Glycosaminoglycan Sequences Through Computerized Modeling. Nehru Viji Sankaranarayanan, Aurijit Sarkar, Umesh R. Desai, and Philip D. Mosier

25.   Using Isothermal Titration Calorimetry To Determine Thermodynamic Parameters Of Protein-Glycosaminoglycan Interactions

26.   Characterizing Protein-Glycosaminoglycan Interactions Using Solution NMR Spectroscopy

Prem Raj B. Joseph, Krishna Mohan Poluri, Krishna Mohan Sepuru, and Krishna Rajarathnam

27.   Glycosaminoglycan–Protein Interaction Studies Using Fluorescence Spectroscopy

Rio S. Boothello, Rami A. Al-Horani, And Umesh R. Desai

28.   Studying Glycosaminoglycan–Protein Interactions Using Capillary Electrophoresis

Aiye Liang and Umesh R. Desai

29.   Histochemical Analysis Of Heparan Sulfate 3-O-Sulfotransferase Expression In Mouse Brain

30.   Keratan Sulfate: Biosynthesis, Structures And Biological Functions

Kenji Uchimura

31.   The Sulfs: Expression, Purification And Substrate Specificity

Kenji Uchimura

32.   The Detection Of Glycosaminoglycans In Pancreatic Islets And Lymphoid Tissues

Marika Bogdani,Charmaine Simeonovic, Nadine Nagy, Pamela Y. Johnson, Christina K. Chan, and Thomas N. Wight

33.   Non-Radioactive Glycosyltransferase And Sulfotransferase Assay To Study Glycosaminoglycan Biosynthesis

Cheryl M Ethen, Miranda Machacek, Brittany Prather, Timothy Tatge, Haixiao Yu, and Zhengliang L Wu

34.   Mapping Proteoglycan Functions With Glycosidases

Mauricio Cortes, Leslie K. Cortes, and Nancy B. Schwartz

35.   Cell Substrate Patterning With Glycosaminoglycans To Study Their Biological Roles In The Central Nervous System. Tony W. Hsiao, Vimal P. Swarup, Colin D. Eichinger, and Vladimir Hlady

36.   Analyzing The Role Of Heparan Sulfate Proteoglycans In Axon Guidance In Vivo In Zebrafish

 Fabienne E. Poulain

37.   Murine Models In The Evaluation Of Heparan Sulfate-Based Anticoagulants

David Gailani, Qiufang Cheng, and Ivan S. Ivanov

38.   Genetic Approaches In The Study Of Heparan Sulfate Functions In Drosophila

Masahiko Takemura and Hiroshi Nakato

39.   Measuring Sulfatase Expression And Invasion In Glioblastoma

Anna Wade, Jane R. Engler, Vy My Tran, and Joanna J. Phillips

40.   Synthesis And Biomedical Applications Of Xylosides

Mausam Kalita, Maritza V. Quintero, Karthik Raman, Vy M. Tran, and Balagurunathan Kuberan

41.   A Strategic Approach To Identification Of Selective Inhibitors Of Cancer Stem Cells

Nirmita Patel, Somesh Baranwal, and Bhaumik B. Patel

42.   Analysis Of The Heavy Chain Modification And Tsg-6 Activity In Pathological Hyaluronan Matrices Mark E. Lauer, Jacqueline Loftis, Carol De La Motte, and Vincent C. Hascall

43.   Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration

Hong Qiu, Wen-Yuan Xiao, Jingwen Yue, and Lianchun Wang

44.   Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions

 James A. Vassie, John M. Whitelock, and Megan S. Lord

45.   Role Of Glycosaminoglycans In Infectious Disease

Akiko Ohno-Jinno and Pyong Woo Park

 Simon J. Foulcer , Anthony J. Day, and Suneel S. Apte

47.   Analysis Of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility In Myofibroblast Differentiation

Adam C. Midgley and Timothy Bowen

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts