Description
Glycosyltransferases, Softcover reprint of the original 1st ed. 2013
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1022
Coordinator: Brockhausen Inka
Language: EnglishSubjects for Glycosyltransferases:
Publication date: 08-2016
Support: Print on demand
Publication date: 06-2013
425 p. · 17.8x25.4 cm · Hardback
Description
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Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease. Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize specific linkages between sugars or sugars and protein. This is in contrast to the non-specific or less specific chemical glycation reactions, transglycosylation and reverse glycosylation reactions. Glycosyltransferases: Methods and Protocols contains a wide range of studies, methods and protocols which form a solid basis for investigations of the role and mechanisms, biology and pathology involving GTs. Written in the successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Glycosyltransferases: Methods and Protocols is a vital contribution to glycobiology and glycopathology, and to applications of these enzymes in biotechnology and drug development. It will prove invaluable to students, postdoctoral fellows, and senior scientists carrying on research of GTs that has been intensified over the last years.
1. Glycan Microarray Screening Assay for Glycosyltransferase Specificities
Wenjie Peng, Corwin M. Nycholat, and Nahid Razi
2. A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-synthase) Activity
Tongzhong Ju and Richard D. Cummings
3. Structural and Biochemical Analysis of a Bacterial Glycosyltransferase
Fan Zhu, Ren Wu, Hua Zhang, and Hui Wu
4. Study of the Biological Functions of Mucin Type Core 3 O-glycans
Seung Ho Lee and Minoru Fukuda
5. Generation of Anti-Sulfated Glycan Antibodies using Sulfotransferase-deficient Mice
Hiroto Kawashima
6. Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells
Rose A. Willett, Irina D. Pokrovskaya, and Vladimir V. Lupashin
7. A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights into the Sequence-Function Relationships of Golgi-Glycosyltransferases
Daniel Petit, Roxana Elin Teppa, Jean-Michel Petit, and Anne Harduin-Lepers
8. Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans during Development
E. Tian, Liping Zhang, and Kelly G. Ten Hagen
9. Photoaffinity Labeling of Protein O-Mannosyltransferases of the PMT1/PMT2 Subfamily
Martin Loibl and Sabine Strahl
10. Enzymatic Analysis of the Protein O-glycosyltransferase, Rumi, Acting toward Epidermal Growth Factor-like (EGF) Repeats
Hideyuki Takeuchi and Robert S. Haltiwanger
11. Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
Eun Ju Kim and John Hanover
12. Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase, and Assay of its Substrate, UDP-GlcNAc
Tony Lefebvre, Ludivine Drougat, Stéphanie Olivier-van Stichelen, Jean-Claude Michalski, and Anne-Sophie Vercoutter-Edouart
13. In vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases
Matias A. Musumeci, Maria V. Ielmini, and Mario F. Feldman
14. In vitro UDP-sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection by Thin Layer Chromatography
Kinnari B. Patel and Miguel A. Valvano
15. In vitro O-Antigen Ligase Assay
Xiang Ruan and Miguel A. Valvano
16. Functional Identification of Bacterial Glucosyltransferase WbdN
Yin Gao, Anna Vinnikova, and Inka Brockhausen
17. Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains
Paul DeAngelis
18. Methods for Measuring Class I Membrane-bound Hyaluronan Synthase Activity
Paul H. Weigel, Amy J. Padgett-McCue, and Bruce A. Baggenstoss
19. Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei
Luis Izquierdo, M. Lucia S. Güther, and Michael A.J. Ferguson
20. Mannose-6-Phosphate: A Regulator of LLO Destruction
Ningguo Gao and Mark A. Lehrman
21. N-Acetylglucosaminyltransferase (GnT) Assays using Fluorescent Oligosaccharide Acceptor Substrates: GnT-III, IV, V, and IX (GnT-Vb)
Shinji Takamatsu, Hiroaki Korekane, Kazuaki Ohtsubo, Suguru Oguri, Jong Yi Park, Akio Matsumoto, and Naoyuki Taniguchi
22. A Method for Determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI Activity
Tomohiko Taguchi and Naoyuki Taniguchi
23. In vitro Assays of Orphan Glycosyltransferases and their Application to Identify Notch Xylosyltransferases
Maya K. Sethi, Falk F. R. Buettner, Angel Ashikov, and Hans Bakker
24. In vitro Folding of -1,4Galactosyltransferase and Polypeptide--N-acetylgalactosaminyltransferase from the Inclusion Bodies
Boopathy Ramakrishnan and Pradman K. Qasba
25. An Assay for 1,6-Fucosyltransferase (FUT8) Activity Based on the HPLC Separation of a Reaction Product with Fluorescence Detection
Hideyuki Ihara, Hiroki Tsukamoto, Naoyuki Taniguchi, and Yoshitaka Ikeda
26. Immunodetection of Glycosyltransferases in Gastrointestinal Tissues
Joana Gomes and Celso A. Reis
27. Glycosyltransferases in Chemo-Enzymatic Synthesis of Oligosaccharides
Boris Tefsen and Irma van Die
28. Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide
Shingo Hatakeyama, Toshiaki K. Shibata, Yuki Tobisawa, Chikara Ohyama, Kazuhiro Sugihara, and Michiko N. Fukuda
29. Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting - SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-step Lectin Affinity Chromatography
Catharina Steentoft, Eric Paul Bennett, and Henrik Clausen
30. Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases
Malene Bech Vester-Christensen, Eric Paul Bennett, Henrik Clausen, and Ulla Mandel
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts