Hydrogen Sulfide in Redox Biology Part A Methods in Enzymology Series

These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant.

HYDROGEN SULFIDE DETECTION METHODS
1. Mechanistic Chemical Perspective of Hydrogen Sulfide Signaling
Péter Nagy
2. Measurement of H2S in vivo and in vitro by the Monobromobimane Method
Xinggui Shen, Gopi K. Kolluru, Shuai Yuan and Christopher Kevil
3. Hydrogen Sulfide Detection Using Nucleophilic Substitution-Cyclization Based Fluorescent Probes
Bo Peng, Ming Xian
4. Azide-Based Fluorescent Probes: Imaging Hydrogen Sulfide in Living Systems
Vivian S. Lin, Alexander R. Lippert and Christopher J. Chang
5. Chemiluminescent Detection of Enzymatically Produced H2S
T. Spencer Bailey and Michael D. Pluth
6. Quantification of Hydrogen Sulfide Concentration Using Methylene Blue and 5,5’-dithiobis(2-nitrobenzoic acid) Methods in Plants
Zhong-Guang Li
7. H2S Analysis in Biological Samples Using Gas Chromatography with Sulfur Chemiluminescence Detection
Victor Vitvitsky and Ruma Banerjee
HYDROGEN SULFIDE DONORS AND THEIR PHARMACOLOGICAL ACTIVITY
8. Use of Phosphorodithioate-Based Compounds as Hydrogen Sulfide Donors
Chung-Min Park and Ming Xian
9. GYY4137, a Novel Water-soluble, H2S-releasing Molecule
Peter Rose, Brian W. Dymock and Philip K. Moore
10. Neuroprotective Effects of Hydrogen Sulfide in Parkinson’s Disease Animal Models: Methods and Protocols
Xue Xue and Jin-Song Bian
HYDROGEN SULFIDE METABOLISM IN MAMMALIAN TISSUES
11. Assay Methods for H2S Biogenesis and Catabolism Enzymes
Ruma Banerjee, Taurai Chiku, Omer Kabil, Marouane Libiad, Nicole Motl and Pramod K. Yadav
12. Oxidation of H2S in Mammalian Cells and Mitochondria
Abbas Abou-Hamdan, Hala Guedouari-Bounihi, Véronique Lenoir, Mireille Andriamihaja,  François Blachier and Frédéric Bouillaud
13. Redox Regulation of Mammalian 3-mercaptopyruvate Sulfurtransferase
Noriyuki Nagahara, Masatoshi Nagano, Takaaki Ito and Hidenori Suzuki
14. Role of Human Sulfide:quinone Oxidoreductase in H2S Metabolism
Michael R. Jackson, Scott L. Melideo and Marilyn Schuman Jorns
15. H2S Regulation of Nitric Oxide Metabolism
Gopi K. Kolluru, Shuai Yuan, Xinggui Shen and Christopher G. Kevil