Induced Pluripotent Stem (iPS) Cells, 1st ed. 2016
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1357

This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocolsguides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols provides protocols that can be used by both experts and novices interested in stem cells.

1. The piggyBac Transposon as a Platform Technology for Somatic Cell Reprogramming Studies in Mouse

Knut Woltjen, Shin-Il Kim, and Andras Nagy

2. Vallier Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus

Filipa AC Soares, Roger A Pedersen, Ludovic

3. Virus-based Reprograming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton’s Jelly into Induced Pluripotent Stem Cells

Cristian Miere, Liani Devito, and Dusko Ilic Sendai

4. Very Rapid and Efficient Generation of Induced Pluripotent Stem Cells from Mouse pre-B Cells

Bruno Di Stefano and Thomas Graf 

5. Generation of iPS cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Ruijun Jeanna Su, Amanda Neises, Xiao-Bing Zhang

6. Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA

Jason Dang and Tariq Rana

7. Generation of Partially Reprogrammed Cells and Fully Reprogrammed iPS Cells By Plasmid Transfection

Jong Soo Kim, Hyun Woo Choi, Yean Ju Hong, Jeong Tae Do

8. Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts to Induced Pluripotent Stem Cells (iPSCs)

Lucas V Greder, Jason Post and James R Dutton

9. Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons

Shin-Il Kim, Fabian Oceguera-Yanez, Chiho Sakurai, Masato Nakagawa, Shinya Yamanaka, and Knut Woltjen

10. Generation and Characterization of Rat iPSCs

Jing Liao, Ph.D.  Cui C.

11. Generation of Induced Pluripotent Stem Cells in Rabbits

Marielle Afanassieff Yann Tapponnier and Pierre Savatier

12. Monkey Induced Pluripotent Stem Cells Generated by Using Allogeneic Genes

13. Induced Pluripotent Stem Cells from Nonhuman Primates

Anuja Mishra, Zhifang Qiu, Steven Farnsworth, Jacob Hemmi, Miao Li, Alex Pickering, and Peter Hornsby 

14. Computational Biology Methods for Characterization of Pluripotent Cells 

Marcos J. Araúzo-Bravo

15. cGMP-compliant Expansion of Human iPSC Cultures as Adherent Monolayers

Patrick Walsh, Vincent Truong and James R Dutton

16. Analysis of the Mitochondrial DNA and its Replicative Capacity in Induced Pluripotent Stem Cells

Gael Cagnone, Vijesh Vaghjiani, William Lee, Claire Sun, Jacqueline Johnson, Ka-Yu Yeung , Justin C. St. John

17. Selection of Phage Display Peptides Targeting Human Pluripotent Stem Cell Derived Progenitor Cell Lines

Paola A. Bignone, Rachel A. Krupa, Michael West, and David Larocca

18. piggyBac Transposon Mediated Reprogramming and Flow Cytometry Analysis of CD44 and ICAM1 Cell-Surface Marker Changes

Sara Brightwell and Keisuke Kaji

19. Generation and in vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells

Ayaka Yanagida, Hiromitsu Nakauchi, and Akihide Kamiya

20. Induced Pluripotent Stem (iPS) Cell Culture Methods and Induction of Differentiation into Endothelial Cells

Ishita Chatterjee, Fei Li, Erin E. Kohler, Jalees Rehman, Asrar B. Malik, Kishore K. Wary

21. Derivation of Neural Stem Cells from Mouse Induced Pluripotent Stem Cells

Işıl Karanfil and Tugba Bagci-Onder

22. Production of Retinal Cells from Confluent Human iPS Cells

Sacha Reichman and Olivier Goureau

23. Differentiation of iPSC to Mesenchymal Stem-Like Cells and their Characterization

Kim Hynes, Danijela Menicanin, Stan Gronthos, Mark P Bartold

Kahoko Umeda, Nobuaki Shiraki, Shoen Kume

25. Determination of Functional Activity of Human iPSC-derived Hepatocytes by Measurement of CYP Metabolism

Pooja Chaudhari  Neha Prasad, Lipeng Tian, and Yoon-Young Jang 

26. Induced Pluripotent Stem Cells: Generation, Characterization and Differentiation –Methods and Protocols

Veronica KonGraversen and Sai H. Chavala

27. Mesoderm Differentiation from hiPS Cells

Hiroyuki Miwa and Takumi Era

28. Enhancing Human Cardiomyocyte Differentiation from Induced Pluripotent Stem Cells with Trichostatin-A

Shiang Y. Lim; Priyadharshini Sivakumaran; Duncan E. Crombie; Gregory J. Dusting;Alice Pébay, and Rodney J. Dilley

29. Derivation of Skeletal Myogenic Precursors from Human Pluripotent  Stem Cells Using Conditional Expression of PAX7

30. Chondrogenic and Osteogenic Induction from iPS Cells

Ji-Yun Ko and Gun-Il Im

31. Generation of iPS Cells from Granulosa Cells

Jian Mao and Lin Liu

32. The Characteristics of Murine iPS Cells and siRNA Transfection under Hypoxia

K. Sugimoto and Y. Hayashi

33. Hepatic Differentiation from Murine and Human iPS cells using Nanofiber Scaffolds

Taiji Yamazoe, Nobuaki Shiraki, and Shoen Kume

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts