Mechanisms in Recombination, Softcover reprint of the original 1st ed. 1974

This book contains the papers presented at the Twenty-Seventh Annual Biology Division Research Conference which was held April 1-4, 1974 in Gatlinburg, Tennessee. The topic of the symposium was Mechanisms in Recombination and it follows by exactly twenty years the previous Gatlinburg Symposium on Genetic Recombination. During this interval, and the preceding years as well, the process of recombination has remained a central and tantalizing problem for geneticists. The subject assumes added significance with the recent appeal by a committee of leading scientists for a moratorium on the construction of certain types of recombinant molecules. That autonomously replicating molecules linking portions of pro­ karyotic and eukaryotic DNA can now be produced in vitro attests to the technical advances that have taken place in this field. Nevertheless, the details underlying the process in vivo continue to be elusive. This symposium brought together individuals studying recombi­ nation in organisms as widely separated as bacteriophage and mammals and using disciplinary approaches of comparable diversity. Conse­ quently the present volume summarizes much of current strategies and concepts concerning the subject. The meeting was sponsored by the Biology Division of the Oak Ridge National Laboratory (operated by the Union Carbide Corporation for the U. S. Atomic Energy Commission) with the support and encour­ agement of its director, H. I. Adler. The organizing committee was chaired by J. K. Setlow and included R. F. Grell, R. D. Hotchkiss and E. Volkin. Special thanks are due to the speakers, to I. R.

Recombination in Bacteria and their Phages.- Exchange of Parental DNA during Genetic Recombination in Bacteriophage ?X174.- Recombination in Bacteriophage f1.- On the Role of Escherichia coli DNA Polymerase I and of T4 Gene 32 Protein in Recombination of Phage T4.- Heterozygotes as Intermediates of Bacteriophage Recombination.- Mapping of Point Mutations on the Physical Map of Coliphage Lambda: Absence of Clustering for Odd-Numbered Exchanges.- Site-Specific Recombination: Genes and Regulation.- Recombination of Phage ? DNA in Vitro.- chi Mutations of Phage Lambda.- In Vitro Studies of the gam Gene Product of Bacteriophage ?.- Transduction of recB- Hosts is Promoted by ? red+ Function.- Transformation and Transduction of Escherichia coli: The Nature of Recombinants Formed by Rec, RecF, and ? Red.- The RecE Pathway of Bacterial Recombination.- Isolation of the Enzyme Associated with the sbcA Indirect Suppressor.- Transformation-Mediated Recombination in Escherichia coli.- Transformation of Escherichia coli by Recombinant Plasmid Replicons Constructed in Vitro.- Uptake and Integration of Transforming DNA in Bacillus subtilis.- In Vitro Transformation in Toluenized Bacillus subtilis.- Transformation in Haemophilus influenzae.- On the Role of Restriction Enzymes of Haemophilus in Transformation and Transfection.- Role of a Deoxyribonuclease in Bacterial Transformation.- Marker Effects in Pneumococcal Transformation.- Recombination in Fungi.- Genetic and Biochemical Studies of Recombination in Ustilago maydis.- Genetical Interference and Gene Conversion.- The Relationship Between Genetic Recombination and Commitment to Chromosomal Segregation at Meiosis.- Properties of Gene Conversion of Deletions in Saccharomyces cerevisiae.- Lack of Correspondence between Genetic and Physical Distances in the iso-1-Cytochrome c Gene of Yeast.- Recombination of Mitochondrial Genes in Yeast.- The Isolation of Mitotic rec- Mutants in Schizosaccharomyces pombe.- Recombination in Higher Eukaryotes.- Intergenic Recombination, DNA Replication and Synaptonemal Complex Formation in the Drosophila Oocyte.- Studies on Recombination in Higher Organisms.- Genic Control of Meiosis and Some Observations on the Synaptonemal Complex in Drosophila melanogaster.- Coincidence of Modified Crossover Distribution with Modified Synaptonemal Complexes.- Synaptonemal Complex Karyotyping in Spreads of Mammalian Spermatocytes.- An Extrachromosomal Suppressor of Male Crossing-Over in Drosophila ananassae.- X-Ray and Ultraviolet Light Sensitivities of a Methyl Methanesulfonate-Sensitive Strain of Drosophila melanogaster.- Models of Recombination.- Red-Mediated Recombination in Bacteriophage Lambda.- A Replicator’s View of Recombination (and Repair).- Concerning the Stereochemistry of Strand Equivalence in Genetic Recombination.- The Evolution of Recombination Models.- Contributors.