Methods in Mouse Atherosclerosis, Softcover reprint of the original 1st ed. 2015 Methods in Molecular Biology Series, Vol. 1339

This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors? personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies.

1. Use of Mouse Models in Atherosclerosis Research

Godfrey S. Getz and Catherine A. Reardon

2. Cytokines and Immune Responses in Murine Atherosclerosis

P.J.H. Kusters and E. Lutgens

3. Intravital Microscopy for Atherosclerosis Research

Remco T. A. Megens and Oliver Soehnlein

4. Non-invasive Molecular Imaging of Mouse Atherosclerosis

Martina A. McAteerand Robin P. Choudhury

5. Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root

M. Jesús Andrés-Manzano, Vicente Andrés, and Beatriz Dorado

6. Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages

Inés Pineda-Torra,Matthew Gage, Alba de Juan, and Oscar M. Pello

7. Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies

Pedro Molina-Sánchez and Vicente Andrés

8. Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay

Ricardo Villa-Bellosta and Magda R. Hamczyk

9. Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta

Prashanthi Menonand Edward A. Fisher

11. Flow Cytometric Analysis of Immune Cells Within Murine Aorta

Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, and Elena V. Galkina

12. In Vitro Differentiation of Naïve CD4⁺ T cells: A Tool for Understanding the Development of Atherosclerosis

Salvador Iborra and Jose M. Gonzalez-Granado

13. Quantification of Apoptosis in Mouse Atherosclerotic Lesions

Nichola L Figg and Martin R Bennett

14. Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions

José J. Fuster

15. Quantification of in vitro Macrophage Cholesterol Efflux and in vivo Macrophage-Specific Reverse Cholesterol Transport

Joan Carles Escolà-Gil, Miriam Lee-Rueckert, David Santos, Lídia Cedó, Francisco Blanco-Vaca, and Josep Julve

16.In vitro Macrophage Phagocytosis Assay

Magda R. Hamczyk, Ricardo Villa-Bellosta, Vicente Andrés

17. Glucose and Insulin Tolerance Tests in the Mouse

Ángela Vinué and Herminia González-Navarro

18. Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries

Lara del Campo and Mercedes Ferrer

19. Pressure Myography to Study the Function and Structure of Isolated Small Arteries

Olav L. Schjørring, Rune Carlsson,  and Ulf Simonsen

20. Generation of Aorta Transcript Atlases of Wild-type and Apolipoprotein E-null Mice by Laser Capture Microdissection-based mRNA Expression Microarrays

21. Analysis of Gene and Protein Expression In Atherosclerotic Mouse Aorta By Western Blot and Quantitative Real-Time PCR

José Rivera-Torres

22. Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis

Vinatha Sreeramkumar and Andrés Hidalgo

23. Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse

Chen Y.C.^, Rivera J., and Peter K.

24. Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions

Judith C. Sluimer, Marion J. Gijbels,andSylvia Heeneman

25. Mechanical Stabilization of Mouse Carotid Artery for in vivo intravital Microscopy Imaging of Atherogenesis

Raphaël Chèvre

26. Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies

Cristina Rius and María J. Sanz

27. Fluorescent Molecular Tomography for in vivo Imaging of Mouse Atherosclerosis

Alicia Arranz, Markus Rudin, Carlos Zaragoza, and Jorge Ripoll

28. In vivo¹⁸F-FDG-PET Imaging in Mouse Atherosclerosis

Jesús Mateo, Izaskun Bilbao, Juan José Vaquero, Jesús Ruiz-Cabello, and Samuel España

29. Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta

Jesús Mateo, Marina Benito, Samuel España, Javier Sanz, Jesús Jiménez-Borreguero Valentín Fuster, and Jesús Ruiz-Cabello

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras