Microbial Toxins, 2011 Methods and Protocols Methods in Molecular Biology Series, Vol. 739

Interest in the field of microbial toxins is ever growing and spreading across a broad spectrum of scientific disciplines.In an effort to supplement the available reference texts on toxins, Microbial Toxins: Methods and Protocols includes protocols on mold fungus toxins, with some focus on aflatoxins. Intended to support a wide variety of researchers, Microbial Toxins: Methods and Protocols presents the reader with biological, chemical, physical, and medical approaches, as well as state-of-the-art research techniques. Divided into three convenient sections, this detailed volume covers bacterial protein toxins, endotoxins, and mold fungus toxins. Written in the highly successful Methods in Molecular Biology? series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Microbial Toxins: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential field.

Bacterial Protein Toxins.- Detection of Bacterial Protein Toxins by Solid Phase Magnetic Immunocapture and Mass Spectrometry. Sensitive and Rapid Detection of Cholera Toxin-Producing Vibrio cholerae using Loop-Mediated Isothermal Amplification. Ultra Sensitive Detection of Botulinum Neurotoxins and Anthrax Lethal Factor in Biological Samples by ALISSA. Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry. A Cell-Based Fluorescent Assay to Detect the Activity of Shiga Toxin and Other Toxins That Inhibit Protein Synthesis. Use of a Vero Cell-Based Fluorescent Assay to Assess Relative Toxicities of Shiga Toxin 2 Subtypes from Escherichia coli. Molecular Methods – Chip Assay and Quantitative Real-Time PCR – in Detecting Hepatotoxic Cyanobacteria. Endotoxins.- Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes from Whole-Cell Lysates. Isolation of Smooth-Type Lipopolysaccharides to Electrophoretic Homogeneity. A Method for Unobtrusive Labeling of Lipopolysaccharides with Quantum Dots. Fluorescence-Based Methods to Assay Inhibitors of Lipopolysaccharide Synthesis. Micromethods for Lipid A Isolation and Structural Characterization. Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides (LPS) with Probes Bearing Hydrazine or Amino Groups. I. LPS Activation with Cyanogen Bromide.Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides (LPS) with Probes Bearing Hydrazine or Amino Groups. II. LPS Activation with a Cyanopyridinium Agent. Mold Fungus Toxins.- Extraction and Analysis of Fumonisins and Compounds Indicative of Fumonisin Exposure in Plant and Mammalian Tissues and Cultured Cells. Determination of Fumonisins B₁ and B₂ in Maize Food Products by a New Analytical MethodBased on High-Performance Liquid Chromatography and Fluorometric Detection with Post-Column Derivatization. A Confirmatory Method for Aflatoxin M₁ Determination in Milk Based on Immunoaffinity Clean-Up and High-Performance Liquid Chromatography with Fluorometric Detection. Simultanious Determination of Aflatoxins B₁, B₂, G₁ and G₂ in Foods and Feed Materials by High-Performance Liquid Chromatography with On-Line Photochemical Derivatization and Fluorescence Detection. Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus.A Rapid Enzymatic Method for Aflatoxin B Detection