Microchip Capillary Electrophoresis Protocols, Softcover reprint of the original 1st ed. 2015
Methods in Molecular Biology Series, Vol. 1274

In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis. Chapters focus on small molecule, biomolecule applications, various detection modes, and sample preparation approaches are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microchip Capillary Electrophoresis Protocol aids scientists in continuing to study microchip capillary electrophoresis.

Part I. Overview

1. An Overview of the use of Microchips in Electrophoretic Separation Techniques: Fabrication, Separation Modes, Sample Preparation Opportunities, and On-Chip Detection

Stijn Hendrickx, Wim De Malsche, and Deirdre Cabooter

Part II. Microchip Capillary Electrophoresis Of Small Molecules

2. Microchip Electrophoresis for Fast and Interference-Free Determination of Trace Amounts of Glyphosate and Glufosinate Residues in Agricultural Products

Xuan Wei And Qiaosheng Pu

3. Microchip Capillary Electrophoresis Of Nitrite and Nitrate in Cerebrospinal Fluid

Marián Masár, Róbert Bodor, and Peter Troška

4. Analysis Of Thiols By Microchip Capillary Electrophoresis for In Situ Planetary Investigations

Maria F. Mora, Amanda M. Stockton, and Peter A. Willis

5. Determination of Ofloxacin in Ofloxacin Ear Drops by Microfluidic Chip with Contactless Conductivity Detection

Bing Chen and Kaicheng Li

Part III. Microchip Capillary Electrophoresis Of Nucleic Acids, Proteins, Peptides, Pathogens

6. Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation

Sona Krizkova, Hoai Viet Nguyen, Maja Stanisavljevic, Pavel Kopel, Marketa Vaculovicova, Vojtech Adam, and Rene Kizek

7. Fast High-Throughput Screening Of H1N1 Virus by Parallel Detection with Multi-Channel Microchip Electrophoresis

Peng Zhang, He Nan, Seungah Lee, and Seong Ho Kang

8. Sex Identification of Ancient DNA Samples using a Microfluidic Device

Kirsty J Shaw, Keri A. Brown, Terence A. Brown, and Stephen J. Haswell

 9. Fast and Continuous-Flow Detection and Separation Of DNA-Complexes and DNA in Nanofluidic Chip Format

Martina Viefhues, Jan Regtmeier, and Dario Anselmetti

Ruige Wu, Zhiping Wang, and Ying Sing Fung

11. Fast Assembly of Anti-Voltage Photonic Crystals in Microfluidic Channels for Ultrafast Separation of Amino Acids and Peptides

Yi Chen, Tao Liao and Can Hu

Part IV. Nonaqueous Applications

12. Rapid Determination of Catecholamines in Urine Samples by Nonaqueous Microchip Electrophoresis with LIF Detection

Hongmei Hu and Yuanming Guo

Part V. Capillary Electrochromatography On Microchips

13. Carbon Nanotube Based Separation Columns for Microchip Electrochromatography

K. B. Mogensen, B. Delacourt, and J. P. Kutter

Y. Ladner, G. Cretier, and K. Faure

Part VI. Sample Preparation Approaches

15. On-Chip Electromembrane Extraction for Monitoring Drug Metabolism in Real Time by Electrospray Ionization Mass Spectrometry

Nickolaj J. Petersen, Henrik Jensen, and Stig Pedersen-Bjergaard

16. Sample Preparation for N-Glycosylation Analysis of Therapeutic Monoclonal Antibodies By Electrophoresis

Ákos Szekrényes, Jan Partyka, Csaba Varadi, Jana Krenkova, Frantisek Foret, and András Guttman

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts