MicroRNAs as Tools in Biopharmaceutical Production, 2012

Coordinator: Barron Niall

Language: English

105,49 €

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Add to cartAdd to cart
MicroRNAs as Tools in Biopharmaceutical Production
Publication date:
126 p. · 15.5x23.5 cm · Broché

105,49 €

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Add to cartAdd to cart
Micrornas as tools in biopharmaceutical production
Publication date:
126 p. · 15.5x23.5 cm · Broché

Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells,  miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

Preface.-1 A general introduction to microRNAs, their investigation and exploitation in CHO cell lines.-2 Biogenesis of Mammalian miRNA.-3 Target prediction algorithms and bioinformatics resources for miRNA studies.-4 The CHO miRNA transcriptome.-5 MicroRNAs as engineering targets - pathway manipulation to impact bioprocess phenotypes.-6 MicroRNAs for Enhancement of CHO Cell Proliferation and Stability Insights from Neuroblastoma Studies.-7 MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production.-8 Manipulating Levels of Specific microRNAs in Mammalian Cells.- Index.
Biology of microRNAs for use as tools Implementation of these molecules to improve processes in the Biopharmceutical field Methods to implement these molecules in the Bioprocess setting Includes supplementary material: sn.pub/extras Includes supplementary material: sn.pub/extras