Neural Progenitor Cells, 2013
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1059

The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing ?no new neuron? doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.

Part I- Culturing Neural Precursor Cells from Primary Tissue

1. Culturing Fetal Precursors Cells Using Free-Floating Serum-Free Conditions

            Andrew Chojnacki and Samuel Weiss

2. In vitro Expansion of Foetal Neural Progenitors as Adherent Cell Lines

            Steven M. Pollard

3. Isolate and Culture Precursor Cells from the Adult Periventricular Area

            Chiara Cavazzin, Margherita Neri, and Angela Gritti

4. Culturing and Expansion of Precursor Cells from the Adult Hippocampus

            Dhanisha J. Jhaveri, Boris W. Prosper, and Perry F. Bartlett

5. Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord

            Jean-Philippe Hugnot

6. Culturing and Expansion of “Clinical Grade” Precursors Cells from the Foetal Human Central Nervous System

            Maurizio Gelati, Daniela Profico, Massimo Projetti-Pensi, Gianmarco Muzi, Giada Sgaravizzi, and Angelo Luigi Vescovie

7. Isolating and Culturing of Precursor Cells from the Adult Human Brain

            Florian A. Siebzehnrubl and Dennis A. Steindler

8. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord

            Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahave, Alain Boularan, Alain Privat, and Jean-Philippe Hugnot

9. Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny

            Hassan Azari

10. Isolation of Adult Stem Cells from the Human Olfactory Mucosa

            François Féron, Chris Perry, Stéphane D. Girard, and Alan Mackay-Sim

Part II. Stem Cells vs Progenitor Cells

11. Enumerating Stem Cell Frequency – Neural Colony Forming Cell Assay

            Sharon A. Louis and Carmen K. H. Mak

Part III. Purification of Stem and Progenitor Cells

12. Flow Cytometry of Neural Cells

            Geoffrey W. Osborne

Part IV. Transplantation of Neural Progenitor Cells: Preclinical models

13. Neonatal Transplant in Hypoxic Injury

            Tong Zheng and Michael D. Weiss

14. Isolation and Purification of Self Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke

            Ricardo L. Azevedo-Pereira and Marcel M. Daadi

15. Transplantation of Foetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson’s Disease

            Lachlan H. Thompson and Clare L. Parish

Part V. In vivo Identification of Neural Precursor Cells

16. Distribution of Neural Precursor Cells in Rodent Brain

            Daniel G. Blackmore and Rodney L. Rietze

17. Identifying Neural Precursor Cells in the Adult Human Brain

            Thomas I.H. Park, Henry J. Waldvogel, Johanna M. Montgomery, Edward W. Mee, Peter S. Bergin, Richard L.M. Faull, Mike Dragunow, and Maurice A. Curtis

Part VI. Clinical Application of Neural Precursor Cell Therapy

18. Clinical Trials for the Treatment of Spinal Cord Injury: Not So Simple

            Alan Mackay-Sim and François Féron

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras