Patch Clamp Techniques, 2012 From Beginning to Advanced Protocols Springer Protocols Handbooks Series

Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.

Ch. 1 Prologue: The Ion Channel

Ch. 2 Patch-Clamp Techniques: General Remarks

Ch. 3 Whole-Cell Patch Method

Ch. 4 Perforated Whole-Cell Patch-Clamp Technique: A User’s Guide

Ch. 5 Methods for Processing and Analyzing Single-Channel Data

Ch. 6 Channel Noise

Ch. 7 Slice Patch Clamp

Ch. 8 Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms

Ch. 9 Analysis of Synaptic Plasticity with the Slice Patch-Clamp Recording Technique

Ch. 10 Patch-Clamp Recordings from Neuronal Dendrites

Ch. 11 In Vivo Blind Patch-Clamp Recording Technique

Ch. 12 Two-Photon Targeted Patch-Clamp Recordings in Vivo

Ch. 13 Transporter Current Measurements

Ch. 14 Giant Patch and Macro Patch

Ch. 15 Pipette Perfusion Technique

Ch. 16 Planar Lipid Bilayer Method for Studying Channel Molecules

Ch. 17 Patch-Clamp Capacitance Measurements

Ch. 18 Brief Guide to Patch-Clamp Current Measurements in Organelle Membranes

Ch. 19 Role of Ion Channels in Plants

Ch. 20 Automated Patch Clamping

Ch. 21 Patch-Clamp Biosensor Method

Ch. 22 Temperature-Evoked Channel Activation: Simultaneous Detection of Ionic Currents and Temperature

Ch. 23 Heterologous Expression Systems and Analyses of Ion Channels

Ch. 24 Patch-Clamp and Single-Cell Reverse Transcription-Polymerase Chain Reaction/Microarray Analysis

Ch. 25 Smart-Patch Technique

Ch. 26 Ion Channel Pore Sizing in Patch-Clamp Experiments

Ch. 27 Digital Recordings of Patch-Clamp Data

Ch. 28 Solutions for Patch-Clamp Experiments